| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MUC15 |
| Uniprot No | Q8N387 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-334aa |
| 氨基酸序列 | MLALAKILLISTLFYSLLSGSHGKENQDINTTQNIAEVFKTMENKPISLESEANLNSDKENITTSNLKASHSPPLNLPNNSHGITDFSSNSSAEHSLGSLKPTSTISTSPPLIHSFVSKVPWNAPIADEDLLPISAHPNATPALSSENFTWSLVNDTVKTPDNSSITVSILSSEPTSPSVTPLIVEPSGWLTTNSDSFTGFTPYQEKTTLQPTLKFTNNSKLFPNTSDPQKENRNTGIVFGAILGAILGVSLLTLVGYLLCGKRKTDSFSHRRLYDDRNEPVLRLDNAPEPYDVSFGNSSYYNPTLNDSAMPESEENARDGIPMDDIPPLRTSV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MUC15重组蛋白的3篇代表性文献摘要(模拟示例，供参考)：
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1. **文献名称**: *"Recombinant MUC15 suppresses tumor metastasis by modulating integrin signaling in colorectal cancer"*
**作者**: Zhang Y, et al. (2018)
**摘要**: 研究通过昆虫表达系统制备重组MUC15胞外域蛋白，发现其通过抑制整合素-β1/FAK通路降低结直肠癌细胞迁移和侵袭能力，提示其作为潜在抗转移治疗分子。
2. **文献名称**: *"Structural characterization of human MUC15 glycoprotein expressed in CHO cells"*
**作者**: Lee S, et al. (2020)
**摘要**: 报道在CHO细胞中重组表达MUC15的全长蛋白，通过质谱分析其N-糖基化修饰位点，并证实其与凝集素家族的相互作用，为解析MUC15的生物学功能提供结构基础。
3. **文献名称**: *"Development of MUC15-specific monoclonal antibodies using mammalian-derived recombinant protein"*
**作者**: Park JH, et al. (2017)
**摘要**: 利用HEK293细胞表达MUC15重组蛋白作为免疫原，成功筛选出高特异性单克隆抗体，验证其在乳腺癌组织中的诊断应用潜力。
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**注**：以上为基于领域知识的模拟文献，实际文献需通过PubMed/Google Scholar以“MUC15 recombinant”等关键词检索获取。部分真实文献可能涉及MUC15在肿瘤微环境或免疫应答中的机制研究。
**Background of MUC15 Recombinant Protein**
MUC15 (Mucin 15) is a transmembrane glycoprotein belonging to the mucin family, characterized by its O-glycosylated extracellular domains. These proteins are integral to cellular protection, lubrication, and signaling at epithelial surfaces. MUC15 is encoded by the *MUC15* gene, located on chromosome 11p14.3 in humans, and is expressed in various tissues, including the gastrointestinal tract, lungs, and reproductive organs. Structurally, it contains a short cytoplasmic tail, a transmembrane region, and an extracellular mucin-like domain rich in serine, threonine, and proline residues, which facilitate extensive glycosylation.
Functionally, MUC15 is implicated in cell adhesion, immune modulation, and apoptosis regulation. It interacts with cell surface receptors and extracellular matrix components, influencing signaling pathways such as EGFR and integrin-mediated cascades. In cancer biology, MUC15 exhibits dual roles: it may act as a tumor suppressor by inhibiting cell proliferation and metastasis in certain contexts (e.g., colorectal cancer) or promote malignancy by enhancing cell survival and drug resistance in others (e.g., hepatocellular carcinoma). This context-dependent behavior is linked to its glycosylation patterns and tissue-specific expression.
Recombinant MUC15 protein is engineered using mammalian expression systems (e.g., CHO or HEK293 cells) to mimic native post-translational modifications, particularly glycosylation. Its production enables mechanistic studies on MUC15’s role in disease, antibody development for diagnostics, and exploration of therapeutic targeting. For instance, recombinant MUC15 serves as an antigen in immunoassays to detect autoantibodies in cancers or inflammatory conditions.
Despite progress, challenges remain in deciphering its precise molecular interactions and glycosylation-dependent functions. Ongoing research aims to clarify its therapeutic potential, particularly in immunotherapy and biomarker discovery, underscoring MUC15’s relevance in both basic and translational medicine.
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