| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOB1A |
| Uniprot No | Q9H8S9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-216aa |
| 氨基酸序列 | SFLFSSRSSKTFKPKKNIPEGSHQYELLKHAEATLGSGNLRQAVMLPEGEDLNEWIAVNTVDFFNQINMLYGTITEFCTEASCPVMSAGPRYEYHWADGTNIKKPIKCSAPKYIDYLMTWVQDQLDDETLFPSKIGVPFPKNFMSVAKTILKRLFRVYAHIYHQHFDSVMQLQEEAHLNTSFKHFIFFVQEFNLIDRRELAPLQELIEKLGSKDR |
| 预测分子量 | 40.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MOB1A重组蛋白相关的文献摘要信息，供参考：
1. **文献名称**: "MOB1 Mediated Hippo Signaling Regulates Ras-Linked Tumor Growth and Metastasis"
**作者**: Li et al.
**摘要**: 该研究通过表达重组MOB1A蛋白，揭示了其在Hippo信号通路中与LATS激酶协同抑制YAP/TAZ活性的机制，并证明MOB1A缺失会促进Ras驱动的肿瘤转移。
2. **文献名称**: "Structural Basis of MOB1 Recognition by the Tumor Suppressor RASSF1A"
**作者**: Guo et al.
**摘要**: 作者利用重组人源MOB1A蛋白进行晶体结构解析，发现其与RASSF1A蛋白的相互作用界面，阐明该复合物在细胞周期调控和凋亡中的分子基础。
3. **文献名称**: "Recombinant MOB1A Protein Enhances the Efficacy of Chemotherapy in Triple-Negative Breast Cancer Models"
**作者**: Chen et al.
**摘要**: 研究通过体外纯化的MOB1A重组蛋白，证明其能恢复Hippo通路活性，抑制肿瘤细胞增殖并增强顺铂对三阴性乳腺癌的治疗敏感性。
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**备注**：以上文献为示例性内容，实际文献需通过PubMed或Web of Science检索。若需具体文献，建议使用关键词 "MOB1A recombinant protein" + "Hippo pathway" 或结合研究领域(如癌症、结构生物学)进行筛选。
MOB1A is a key regulatory protein involved in the highly conserved Hippo signaling pathway, which plays a critical role in controlling organ size, tissue homeostasis, and tumor suppression by regulating cell proliferation, apoptosis, and stem cell self-renewal. As a member of the MOB (Mps One Binder) protein family, MOB1A functions as a scaffold and adaptor protein, interacting with core kinases of the Hippo pathway, including MST1/2 and LATS1/2. Its activation through phosphorylation by upstream kinases enables it to bind and activate LATS1/2. which subsequently phosphorylate downstream effectors like YAP/TAZ to inhibit their nuclear translocation and transcriptional activity.
Recombinant MOB1A protein is produced using genetic engineering techniques, typically expressed in bacterial or mammalian systems to ensure proper post-translational modifications. This engineered form retains the functional domains required for kinase interactions, making it a valuable tool for in vitro studies. Researchers utilize recombinant MOB1A to investigate Hippo pathway dynamics, including kinase cascade mechanisms, protein-protein interactions, and structural analyses. Its applications extend to cancer research, particularly in studying tumorigenesis linked to Hippo pathway dysregulation, as well as in drug discovery for identifying potential therapeutic targets.
The development of recombinant MOB1A has advanced understanding of cellular signaling cross-talk, as the Hippo pathway intersects with other pathways such as Wnt and TGF-β. Additionally, it serves as a critical reagent in biochemical assays, including phosphorylation studies and inhibitor screenings. Its role in maintaining genomic stability and regulating the mitotic exit network further underscores its importance in cell cycle research. By providing a standardized, high-purity protein source, recombinant MOB1A accelerates both basic and translational studies in developmental biology, oncology, and regenerative medicine.
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