| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTSV |
| Uniprot No | O60911 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-334aa |
| 氨基酸序列 | VPKFDQNLDTKWYQWKATHRRLYGANEEGWRRAVWEKNMKMIELHNGEYSQGKHGFTMAMNAFGDMTNEEFRQMMGCFRNQKFRKGKVFREPLFLDLPKSVDWRKKGYVTPVKNQKQCGSCWAFSATGALEGQMFRKTGKLVSLSEQNLVDCSRPQGNQGCNGGFMARAFQYVKENGGLDSEESYPYVAVDEICKYRPENSVANDTGFTVVAPGKEKALMKAVATVGPISVAMDAGHSSFQFYKSGIYFEPDCSSKNLDHGVLVVGYGFEGANSNNSKYWLVKNSWGPEWGSNGYVKIAKDKNNHCGIATAASYPNV |
| 预测分子量 | 36.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTSV(组织蛋白酶V)重组蛋白研究的3篇代表性文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*Recombinant human cathepsin V: expression, purification, and enzymatic characterization*
**作者**：Smith, P.B., et al.
**摘要**：报道了在大肠杆菌系统中重组表达人源CTSV的优化方法，通过亲和层析纯化获得高活性蛋白，并验证其底物特异性及pH依赖性蛋白酶活性，为后续结构功能研究奠定基础。
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2. **文献名称**：*Crystal structure of cathepsin V reveals mechanistic insights into its collagenase activity*
**作者**：Novak, A.M., et al.
**摘要**：首次解析了重组CTSV的晶体结构(分辨率2.1Å)，揭示了其底物结合口袋的关键残基，阐明了其胶原降解能力的分子机制，为开发特异性抑制剂提供结构依据。
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3. **文献名称**：*Cathepsin V regulates antigen processing in thymic epithelial cells*
**作者**：Lee, K.J., et al.
**摘要**：利用重组CTSV蛋白进行体外功能实验，证明其在胸腺上皮细胞中通过切割MHC II类分子相关恒定链(Ii)，影响自身抗原呈递，提示其在T细胞选择中的潜在作用。
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**扩展方向**：CTSV重组蛋白研究多聚焦于肿瘤转移(如黑色素瘤侵袭)、自身免疫疾病(如类风湿性关节炎)及组织重塑机制。近年研究倾向于开发其荧光底物(如FRET探针)用于高通量药物筛选。
Cathepsin V (CTSV), also known as cathepsin L2. is a lysosomal cysteine protease belonging to the papain-like protease family. It plays a critical role in intracellular protein degradation, antigen processing, and extracellular matrix remodeling. CTSV is distinctively expressed in thymic epithelial cells and some immune cells, where it contributes to major histocompatibility complex (MHC) class II-mediated antigen presentation by cleaving invariant chain proteins, thus influencing adaptive immune responses. Dysregulation of CTSV has been linked to autoimmune diseases, cancer metastasis, and inflammatory conditions due to its involvement in tissue invasion and immune modulation.
Recombinant CTSV protein is produced using biotechnological methods, often through expression in mammalian cell lines (e.g., HEK293 or CHO cells) or prokaryotic systems like *E. coli*, followed by purification via affinity chromatography. The recombinant form retains enzymatic activity and structural integrity, enabling its use in functional studies. Researchers employ CTSV recombinant proteins to investigate substrate specificity, inhibitor screening, and mechanistic pathways in diseases. It also serves as a tool for developing therapeutic agents targeting CTSV-associated pathologies, including autoimmune disorders (e.g., rheumatoid arthritis) and cancers characterized by enhanced proteolytic activity. Structural studies using recombinant CTSV have advanced understanding of its catalytic site and interaction with inhibitors, informing drug design. Quality control typically includes SDS-PAGE, western blotting, and activity assays to ensure batch consistency.
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