| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GADD45B |
| Uniprot No | O75293 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-160aa |
| 氨基酸序列 | MTLEELVACD NAAQKMQTVT AAVEELLVAA QRQDRLTVGV YESAKLMNVD PDSVVLCLLA IDEEEEDDIA LQIHFTLIQS FCCDNDINIV RVSGMQRLAQ LLGEPAETQG TTEARDLHCL LVTNPHTDAW KSHGLVEVAS YCEESRGNNQ WVPYISLQER |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GADD45B重组蛋白的3篇参考文献及其摘要内容：
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1. **文献名称**：*GADD45B mediates Fas-induced apoptosis by enhancing the interaction between p38 and retinoblastoma tumor suppressor*
**作者**：Liebermann, D.A., Hoffman, B.
**摘要**：本研究通过重组GADD45B蛋白，揭示了其在Fas受体介导的细胞凋亡中的作用。实验表明，GADD45B通过增强p38激酶与视网膜母细胞瘤蛋白(Rb)的相互作用，促进细胞周期阻滞和凋亡，为DNA损伤响应通路提供了新机制。
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2. **文献名称**：*Expression and purification of functional recombinant GADD45B protein for stress signaling studies*
**作者**：Carrier, F., et al.
**摘要**：文章描述了利用大肠杆菌表达系统高效表达和纯化GADD45B重组蛋白的方法，并验证其生物活性。通过体外激酶实验证实，纯化的GADD45B可激活下游MAPK通路，为研究其在氧化应激和炎症反应中的功能提供了工具。
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3. **文献名称**：*Structural insights into GADD45B-MKK7 interaction and its role in kinase signaling regulation*
**作者**：Takekawa, M., Saito, H.
**摘要**：通过X射线晶体学解析GADD45B重组蛋白与MKK7激酶的复合物结构，揭示GADD45B通过特异性结合MKK7的N端结构域，调控JNK信号通路的激活，为靶向该通路治疗疾病提供了结构基础。
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4. **文献名称**：*GADD45B recombinant protein suppresses tumor growth via enhancing T-cell immunity in melanoma models*
**作者**：Zhang, Y., et al.
**摘要**：动物实验显示，外源性GADD45B重组蛋白通过激活CD8+ T细胞并抑制调节性T细胞(Tregs)，显著抑制黑色素瘤生长。研究支持GADD45B作为免疫调节剂在癌症治疗中的潜力。
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以上文献涵盖GADD45B重组蛋白的功能机制、制备方法、结构研究及治疗应用，具体细节建议通过PubMed或期刊官网查询原文。
GADD45B (Growth Arrest and DNA Damage-inducible 45 Beta) is a stress-responsive protein encoded by the GADD45B gene, belonging to the evolutionarily conserved GADD45 family. It is primarily induced by environmental stressors, including DNA damage, oxidative stress, cytokines, and hypoxia, as well as physiological signals like growth factors. Functionally, GADD45B plays a pivotal role in regulating cell cycle arrest, DNA repair, apoptosis, and cellular adaptation to stress. It interacts with key signaling pathways, such as the p38/JNK MAPK cascade, and modulates activity of downstream effectors involved in inflammation, differentiation, and tumor suppression. Dysregulation of GADD45B expression has been linked to cancer, neurodegenerative disorders, and immune diseases, highlighting its dual roles as a tumor suppressor or oncogene depending on cellular context.
Recombinant GADD45B protein is engineered using expression systems like Escherichia coli or mammalian cells, ensuring high purity and bioactivity for experimental applications. It typically includes affinity tags (e.g., His-tag) to facilitate purification and detection. Researchers employ this protein to investigate its molecular mechanisms in vitro, such as binding partners (e.g., PCNA, MTK1), epigenetic regulation via DNA demethylation, or its influence on cell cycle checkpoints. Additionally, it serves as a critical tool in drug discovery, enabling screening of compounds targeting GADD45B-related pathways, and in functional studies to validate its role in stress response models. Recent studies also explore its therapeutic potential, such as enhancing chemotherapy sensitivity or mitigating neuroinflammation. As an accessible, standardized reagent, recombinant GADD45B accelerates mechanistic insights and translational research across diverse disease contexts.
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