| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PFKFB1 |
| Uniprot No | P16118 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-471aa |
| 氨基酸序列 | SPEMGELTQ TRLQKIWIPH SSGSSRLQRR RGSSIPQFTN SPTMVIMVGL PARGKTYIST KLTRYLNWIG TPTKVFNLGQ YRREAVSYKN YEFFLPDNME ALQIRKQCAL AALKDVHNYL SHEEGHVAVF DATNTTRERR SLILQFAKEH GYKVFFIESI CNDPGIIAEN IRQVKLGSPD YIDCDREKVL EDFLKRIECY EVNYQPLDEE LDSHLSYIKI FDVGTRYMVN RVQDHIQSRT VYYLMNIHVT PRSIYLCRHG ESELNIRGRI GGDSGLSVRG KQYAYALANF IQSQGISSLK VWTSHMKRTI QTAEALGVPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF ALRDQDKYRY RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL LAYFLDKSSD ELPYLKCPLH TVLKLTPVAY GCKVESIYLN VEAVNTHREK PENVDITREP EEALDTVPAH Y |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PFKFB1重组蛋白的3篇参考文献概览：
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1. **文献名称**: *"Cloning, expression, and characterization of human PFKFB1: a key enzyme in glucose metabolism regulation"*
**作者**: Okar DA et al.
**摘要**: 该研究报道了人源PFKFB1基因的克隆及其在大肠杆菌中的重组表达，纯化后的蛋白表现出双功能酶活性(激酶和磷酸酶)，并揭示了果糖-2.6-二磷酸对糖酵解通路的调控机制。
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2. **文献名称**: *"Structural insights into the bifunctional activity of PFKFB1 through recombinant protein crystallography"*
**作者**: Kim JW et al.
**摘要**: 通过重组PFKFB1蛋白的结晶和结构解析，阐明了其激酶和磷酸酶结构域的构象变化，解释了pH和底物浓度对酶活性的调节作用。
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3. **文献名称**: *"Functional analysis of PFKFB1 isoforms: Role of recombinant proteins in cancer cell metabolism"*
**作者**: Clem B et al.
**摘要**: 研究利用哺乳动物细胞表达系统制备不同PFKFB1重组蛋白亚型，发现其在肿瘤细胞中通过调控果糖-2.6-二磷酸水平促进糖酵解，影响细胞增殖和存活。
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注：以上文献信息为示例，实际引用时需核实具体来源及细节。建议通过PubMed或Web of Science以“PFKFB1 recombinant”为关键词检索最新研究。
PFKFB1 (6-phosphofructo-2-kinase/fructose-2.6-biphosphatase 1) is a bifunctional enzyme central to cellular energy metabolism. It regulates the synthesis and degradation of fructose-2.6-bisphosphate (F2.6BP), a key allosteric activator of glycolysis and inhibitor of gluconeogenesis. By dynamically balancing F2.6BP levels, PFKFB1 fine-tunes the switch between glycolytic and gluconeogenic pathways, adapting to cellular energy demands. The enzyme contains both kinase (synthesis) and phosphatase (degradation) domains, with its activity influenced by tissue-specific isoforms and post-translational modifications.
Recombinant PFKFB1 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional enzyme for research. Its recombinant form enables detailed studies of enzymatic kinetics, structural properties, and regulatory mechanisms. Researchers utilize it to investigate metabolic disorders, cancer metabolism (where glycolysis often dominates), and insulin signaling pathways. PFKFB1's role in conditions like diabetes, obesity, and cardiovascular diseases has driven interest in developing modulators of its activity, with recombinant protein serving as a critical tool for drug screening and target validation.
Notably, PFKFB1 differs from other PFKFB isoforms (PFKFB2-4) in tissue distribution and regulatory features. While primarily cytosolic, its subcellular localization and stress-responsive expression patterns add complexity to its metabolic functions. Recent studies also suggest non-metabolic roles in cell cycle regulation and apoptosis. The availability of recombinant PFKFB1 continues to advance our understanding of metabolic flexibility in health and disease.
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