| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FSHb |
| Uniprot No | P01225 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-129aa |
| 氨基酸序列 | MKTLQFFFLFCCWKAICCNSCELTNITIAIEKEECRFCISINTTWCAGYCYTRDLVYKDPARPKIQKTCTFKELVYETVRVPGCAHHADSLYTYPVATQCHCGKCDSDSTDCTVRGLGPSYCSFGEMKE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FSHb重组蛋白的3篇代表性文献的简要信息(注：内容基于模拟文献，非真实存在)：
1. **文献名称**：*Expression and Purification of Recombinant FSHb in Mammalian Cells*
**作者**：Zhang Y, et al.
**摘要**：研究通过哺乳动物细胞表达系统(如CHO细胞)高效表达人FSHβ亚基(FSHb)，并优化纯化工艺，获得高纯度、具有生物活性的重组蛋白，为后续临床应用奠定基础。
2. **文献名称**：*Structural Characterization of FSHb by X-ray Crystallography*
**作者**：Smith JL, et al.
**摘要**：利用X射线晶体学解析了重组FSHb的三维结构，揭示了其与受体结合的潜在表位，为设计靶向FSH通路的药物提供了结构生物学依据。
3. **文献名称**：*Functional Analysis of Recombinant FSHb in Ovarian Follicle Development*
**作者**：Brown K, et al.
**摘要**：通过动物模型验证重组FSHb蛋白在促进卵泡发育和调节雌激素分泌中的功能，证明其在不孕症治疗中的潜在应用价值。
如需具体文献，建议在PubMed或Sci-Hub中检索关键词“recombinant FSH beta subunit”或“FSHb protein expression”。
Follicle-stimulating hormone beta subunit (FSHb) recombinant protein is a biologically engineered version of the β-subunit of follicle-stimulating hormone (FSH), a critical glycoprotein hormone involved in reproductive physiology. Naturally produced by the anterior pituitary gland, FSH regulates gametogenesis and follicular development in females and spermatogenesis in males. The functional FSH hormone is a heterodimer consisting of an α-subunit (shared with other hormones like LH and TSH) and a unique β-subunit that confers biological specificity. Recombinant FSHb is produced using genetic engineering techniques, often in mammalian cell systems (e.g., CHO or HEK293 cells) or microbial platforms, ensuring high purity and consistency compared to urinary-derived FSH products.
The development of recombinant FSHb stems from the need for standardized, scalable, and ethical alternatives to human-derived FSH for clinical and research applications. It has become pivotal in treating infertility, particularly in controlled ovarian stimulation for assisted reproductive technologies (ART) like IVF. Recombinant FSHb-based therapies offer precise dosing, reduced batch-to-batch variability, and minimized risk of contaminants. Beyond therapeutics, FSHb recombinant proteins are essential tools in studying FSH receptor interactions, hormone signaling pathways, and reproductive disorders. Recent advancements in protein engineering have also enabled the design of long-acting FSH analogs or modified variants with enhanced stability or targeted activity. Ongoing research explores its potential in veterinary medicine, regenerative therapies, and as a biomarker in reproductive health monitoring.
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