| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CA5B |
| Uniprot No | Q9Y2D0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-317aa |
| 氨基酸序列 | CSLYTCT YKTRNRALHP LWESVDLVPG GDRQSPINIR WRDSVYDPGL KPLTISYDPA TCLHVWNNGY SFLVEFEDST DKSVIKGGPL EHNYRLKQFH FHWGAIDAWG SEHTVDSKCF PAELHLVHWN AVRFENFEDA ALEENGLAVI GVFLKLGKHH KELQKLVDTL PSIKHKDALV EFGSFDPSCL MPTCPDYWTY SGSLTTPPLS ESVTWIIKKQ PVEVDHDQLE QFRTLLFTSE GEKEKRMVDN FRPLQPLMNR TVRSSFRHDY VLNVQAKPKP ATSQATP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CA5B重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
1. **标题**：Cloning and Expression of Human Carbonic Anhydrase VB (CA5B) in Escherichia coli
**作者**：Smith A, et al.
**摘要**：报道了人源CA5B基因的克隆及在大肠杆菌中的重组表达，优化了表达条件并验证了重组蛋白的二氧化碳水合酶活性，为后续功能研究提供基础。
2. **标题**：Structural Characterization of Recombinant CA5B and Its Role in Mitochondrial Dysfunction
**作者**：Lee H, et al.
**摘要**：通过X射线晶体学解析了重组CA5B蛋白的三维结构，发现其在线粒体能量代谢中的调控作用，并与遗传性代谢疾病相关突变位点关联。
3. **标题**：Functional Analysis of CA5B Knockout Mice Using Recombinant Protein Complementation
**作者**：Wang Y, et al.
**摘要**：利用重组CA5B蛋白回补CA5B基因敲除小鼠模型，证实了该酶在肝脏尿素循环中的关键功能及与高氨血症的病理相关性。
(如需真实文献，建议通过PubMed或SciFinder以“CA5B recombinant protein”“carbonic anhydrase 5B expression”为关键词检索。)
CA5B, or Carbonic Anhydrase 5B, is a mitochondrial enzyme belonging to the carbonic anhydrase (CA) family, which catalyzes the reversible hydration of carbon dioxide (CO₂) to bicarbonate (HCO₃⁻) and protons (H⁺). This zinc metalloenzyme plays a critical role in regulating cellular pH, mitochondrial metabolism, and biosynthetic processes such as gluconeogenesis and ureagenesis. The CA5B gene encodes a 317-amino-acid protein primarily expressed in the liver and kidneys, reflecting its importance in systemic acid-base homeostasis and energy metabolism. Unlike other CA isoforms, CA5B’s mitochondrial localization links it to metabolic pathways requiring bicarbonate, including lipogenesis and the urea cycle.
Recombinant CA5B protein is produced using biotechnological platforms, such as bacterial (E. coli) or mammalian expression systems, enabling large-scale purification for research and therapeutic applications. Its recombinant form retains enzymatic activity, making it valuable for studying mitochondrial dysfunction, metabolic disorders (e.g., hyperammonemia), and diseases associated with CA dysregulation, such as cancer and neurodegenerative conditions. Researchers also utilize recombinant CA5B to screen CA-specific inhibitors, which have potential as diuretics, antiepileptics, or anticancer agents. Structural studies of recombinant CA5B aid in understanding its catalytic mechanism and designing isoform-selective drugs to minimize off-target effects. Furthermore, its role in CO₂ metabolism has implications for bioengineering applications, including carbon capture technologies. Despite its physiological significance, CA5B’s specific regulatory mechanisms and interactions within mitochondrial networks remain under investigation, highlighting the need for further exploration using recombinant protein tools. Overall, CA5B recombinant protein serves as a vital resource for both basic research and translational medicine.
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