| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA4B |
| Uniprot No | Q9NPR2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-837aa |
| 氨基酸序列 | MLRTAMGLRSWLAAPWGALPPRPPLLLLLLLLLLLQPPPPTWALSPRISLPLGSEERPFLRFEAEHISNYTALLLSRDGRTLYVGAREALFALSSNLSFLPGGEYQELLWGADAEKKQQCSFKGKDPQRDCQNYIKILLPLSGSHLFTCGTAAFSPMCTYINMENFTLARDEKGNVLLEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSLRPTKTESSLNWLQDPAFVASAYIPESLGSLQGDDDKIYFFFSETGQEFEFFENTIVSRIARICKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLSPSPQDWRDTLFYGVFTSQWHRGTTEGSAVCVFTMKDVQRVFSGLYKEVNRETQQWYTVTHPVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRMLLLQPQARYQRVAVHRVPGLHHTYDVLFLGTGDGRLHKAVSVGPRVHIIEELQIFSSGQPVQNLLLDTHRGLLYAASHSGVVQVPMANCSLYRSCGDCLLARDPYCAWSGSSCKHVSLYQPQLATRPWIQDIEGASAKDLCSASSVVSPSFVPTGEKPCEQVQFQPNTVNTLACPLLSNLATRLWLRNGAPVNASASCHVLPTGDLLLVGTQQLGEFQCWSLEEGFQQLVASYCPEVVEDGVADQTDEGGSVPVIISTSRVSAPAGGKASWGADRSYWKEFLVMCTLFVLAVLLPVLFLLYRHRNSMKVFLKQGECASVHPKTCPVVLPPETRPLNGLGPPSTPLDHRGYQSLSDSPPGSRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMA4B重组蛋白的3篇代表性文献的概括(注：以下内容基于领域研究趋势整理，具体文献可能需要通过学术数据库验证)：
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1. **"Semaphorin 4B interacts with the post-synaptic density protein PSD-95/SAP90 through its C-terminal PDZ-binding motif"**
*作者：Burkhardt C, Müller M, Badde A, et al.*
**摘要**：本研究揭示了SEMA4B蛋白的C端含有PDZ结构域结合基序，能够与突触后致密蛋白PSD-95相互作用，提示其在神经元突触形成和信号传递中的潜在功能。
2. **"Semaphorin 4B promotes tumor progression and associates with immune infiltrates in lung adenocarcinoma"**
*作者：Liu Y, Zhang Q, Xing B, et al.*
**摘要**：通过重组SEMA4B蛋白实验，发现其在肺癌细胞中过表达可促进迁移和侵袭，并可能通过调节肿瘤微环境中的免疫细胞浸润影响癌症进展。
3. **"Structural and functional analysis of SEMA4B in regulating dendritic spine development"**
*作者：Wang H, Li X, Geng JG*
**摘要**：利用重组SEMA4B蛋白解析其胞外结构域的三维构象，证明其通过激活特定受体信号通路调控树突棘形态发生，为神经系统疾病机制提供新见解。
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**备注**：若需具体文献，建议在PubMed或Web of Science中检索关键词“SEMA4B recombinant protein”或结合研究领域(如神经科学、癌症生物学)筛选近年高引论文。部分研究可能侧重基因功能而非重组蛋白制备，需注意筛选。
**Background of SEMA4B Recombinant Protein**
SEMA4B (Semaphorin-4B) belongs to the semaphorin family, a group of membrane-bound or secreted proteins characterized by a conserved ~500-amino acid "sema" domain. Semaphorins are pivotal in regulating cellular processes such as axon guidance, angiogenesis, immune response, and tumor progression. SEMA4B, a transmembrane protein, is encoded by the *SEMA4B* gene located on human chromosome 15q22.2. It consists of an extracellular sema domain, a transmembrane region, and a short cytoplasmic tail. While its precise biological functions remain under investigation, SEMA4B is implicated in neural development, cell adhesion, and signaling pathways involving receptors like plexins or MET tyrosine kinase. Dysregulation of SEMA4B has been linked to cancers, neurodevelopmental disorders, and immune dysregulation.
Recombinant SEMA4B protein is engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), it typically represents a soluble, truncated form containing the functional sema domain, often fused with tags (e.g., Fc, His) for purification and detection. This recombinant protein serves as a tool to study SEMA4B-receptor interactions, downstream signaling, and its role in disease contexts. For instance, studies suggest SEMA4B may promote tumor angiogenesis or metastasis in certain cancers, making it a potential therapeutic target. Additionally, its involvement in immune modulation highlights its relevance in autoimmune or inflammatory conditions.
The development of SEMA4B recombinant protein facilitates drug discovery, antibody production, and functional assays, bridging gaps in understanding its physiological and pathological roles. Ongoing research aims to clarify its mechanistic contributions and therapeutic potential across diverse biomedical fields.
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