| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCLY |
| Uniprot No | Q96I15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MEAAVAPGRD APAPAASQPS GCGKHNSPER KVYMDYNATT PLEPEVIQAM TKAMWEAWGN PSSPYSAGRK AKDIINAARE SLAKMIGGKP QDIIFTSGGT ESNNLVIHSV VKHFHANQTS KGHTGGHHSP VKGAKPHFIT SSVEHDSIRL PLEHLVEEQV AAVTFVPVSK VSGQAEVDDI LAAVRPTTRL VTIMLANNET GIVMPVPEIS QRIKALNQER VAAGLPPILV HTDAAQALGK QRVDVEDLGV DFLTIVGHKF YGPRIGALYI RGLGEFTPLY PMLFGGGQER NFRPGTENTP MIAGLGKAAE LVTQNCEAYE AHMRDVRDYL EERLEAEFGQ KRIHLNSQFP GTQRLPNTCN FSIRGPRLQG HVVLAQCRVL MASVGAACHS DHGDQPSPVL LSYGVPFDVA RNALRLSVGR STTRAEVDLV VQDLKQAVAQ LEDQA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCLY重组蛋白的参考文献示例，涵盖表达、结构及功能研究：
1. **"Recombinant Expression and Characterization of Human Selenocysteine Lyase in E. coli"**
*作者：Smith J, et al.*
摘要：研究成功在大肠杆菌中表达了重组人SCLY蛋白，优化了纯化流程，并验证了其酶活性和对硒代半胱氨酸的特异性裂解功能。
2. **"Crystal Structure of Selenocysteine Lyase Reveals a Novel Catalytic Mechanism"**
*作者：Tanaka M, et al.*
摘要：通过X射线晶体学解析了重组小鼠SCLY的三维结构，揭示了其依赖吡哆醛磷酸(PLP)的催化机制及底物结合位点特征。
3. **"Functional Analysis of Recombinant SCLY in Cellular Selenium Homeostasis"**
*作者：Wang L, et al.*
摘要：利用体外重组SCLY蛋白进行酶动力学研究，证明其在硒代谢中的关键作用，并发现其活性受细胞内氧化还原状态调控。
4. **"Site-Directed Mutagenesis of Recombinant SCLY Highlights Critical Residues for Enzyme Activity"**
*作者：Garcia R, et al.*
摘要：通过构建SCLY点突变体重组蛋白，鉴定了多个对催化活性至关重要的氨基酸残基，为相关遗传性硒代谢疾病的机制研究提供依据。
(注：以上文献为示例性概括，实际引用需根据真实研究调整。)
**Background of SCLY Recombinant Protein**
Selenocysteine lyase (SCLY) is a pyridoxal 5′-phosphate (PLP)-dependent enzyme that catalyzes the decomposition of selenocysteine into alanine and elemental selenium, playing a critical role in selenium metabolism. Selenium, an essential trace element, is incorporated into selenoproteins as selenocysteine (Sec), a process vital for antioxidant defense, thyroid hormone metabolism, and redox signaling. SCLY ensures the recycling of selenium by releasing it from degraded selenoproteins, making it available for synthesizing new selenocysteine residues. Dysregulation of SCLY has been linked to selenium deficiency-related disorders, neurodegenerative diseases, and cancer, underscoring its physiological importance.
The recombinant SCLY protein is produced using genetic engineering techniques, typically by expressing the *SCLY* gene in heterologous systems like *E. coli* or mammalian cell cultures. This approach enables large-scale production of highly pure, functional SCLY for research and therapeutic applications. Recombinant SCLY retains enzymatic activity, allowing scientists to study its structure-function relationships, catalytic mechanisms, and interactions with substrates or inhibitors in vitro. It is also utilized to investigate selenium homeostasis pathways and develop therapies targeting selenium metabolism abnormalities.
Recent studies highlight SCLY's dual role in both selenium recycling and reactive oxygen species (ROS) regulation, linking it to oxidative stress-associated conditions. Additionally, structural analyses of recombinant SCLY have provided insights into its PLP-binding domain and active-site architecture, facilitating drug design. As a tool in biomedical research, recombinant SCLY aids in exploring selenium’s role in health and disease, offering potential for diagnostics or treatments addressing selenium dysregulation.
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