| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Calmegin |
| Uniprot No | O14967 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-610aa |
| 氨基酸序列 | EFMDDDVETEDFEENSEEIDVNESELSSEIKYKTPQPIGEVYFAETFDSGRLAGWVLSKAKKDDMDEEISIYDGRWEIEELKENQVPGDRGLVLKSRAKHHAISAVLAKPFIFADKPLIVQYEVNFQDGIDCGGAYIKLLADTDDLILENFYDKTSYIIMFGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKKKHKDTEYKKTDICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Calmegin重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，实际文献需根据具体研究查询验证):
1. **文献名称**: *Molecular Cloning and Characterization of Calmegin: A Novel Calcium-Binding Protein Expressed in Spermatogenic Cells*
**作者**: Ikawa M, et al.
**摘要**: 首次克隆并鉴定了小鼠Calmegin基因，发现其编码一种睾丸特异性钙结合蛋白，重组蛋白表达证实其参与精子发生过程中的细胞黏附与信号转导。
2. **文献名称**: *Recombinant Calmegin Promotes Sperm-Zona Pellucida Binding in Vitro*
**作者**: Nakanishi T, et al.
**摘要**: 通过昆虫细胞系统表达重组Calmegin蛋白，证明其作为分子伴侣参与精子表面蛋白的正确折叠，并在体外实验中显著增强精子与透明带的结合能力。
3. **文献名称**: *Structural Insights into Calmegin’s Role in Fertility via Cryo-EM Analysis*
**作者**: Chen Z, et al.
**摘要**: 利用冷冻电镜解析重组人源Calmegin蛋白的高分辨率结构，揭示其与卵子表面配体相互作用的特异性结构域，为男性不育机制提供分子基础。
(提示：实际引用时建议通过PubMed/Google Scholar检索最新文献，并使用真实存在的论文信息。)
**Background of Calmegin Recombinant Protein**
Calmegin (CLGN) is a testis-enriched chaperone protein belonging to the calnexin family, which plays a critical role in protein quality control during spermatogenesis. Structurally, it contains a conserved lectin-like domain that facilitates binding to glycoproteins, ensuring proper folding and maturation of client proteins. Unlike its homolog calnexin, calmegin is predominantly expressed in male germ cells, where it supports the development of functional spermatozoa by mediating the assembly of sperm-specific surface proteins and adhesion molecules.
In female reproduction, calmegin is also detected in oocytes, suggesting a broader role in gametogenesis. Studies highlight its involvement in sperm-egg recognition and fusion, as calmegin-deficient mice exhibit impaired fertility due to defective sperm-zona pellucida binding. This underscores its importance in fertilization processes.
Recombinant calmegin is engineered using heterologous expression systems (e.g., mammalian or insect cells) to produce soluble, functional protein for *in vitro* studies. Its recombinant form retains the ability to bind glycoproteins, enabling researchers to dissect molecular interactions critical for gamete maturation and fertilization. Applications include elucidating infertility mechanisms, developing diagnostic biomarkers, and screening compounds targeting reproductive disorders.
The production of recombinant calmegin overcomes challenges associated with isolating native protein from tissues, offering high purity and consistency. It serves as a vital tool for structural studies, antibody development, and functional assays, advancing reproductive biology and therapeutic research.
In summary, calmegen recombinant protein bridges gaps in understanding molecular chaperones in reproduction, providing insights into fertility and potential clinical applications.
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