| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRCP |
| Uniprot No | P42785 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 46-496aa |
| 氨基酸序列 | KNYSV LYFQQKVDHF GFNTVKTFNQ RYLVADKYWK KNGGSILFYT GNEGDIIWFC NNTGFMWDVA EELKAMLVFA EHRYYGESLP FGDNSFKDSR HLNFLTSEQA LADFAELIKH LKRTIPGAEN QPVIAIGGSY GGMLAAWFRM KYPHMVVGAL AASAPIWQFE DLVPCGVFMK IVTTDFRKSG PHCSESIHRS WDAINRLSNT GSGLQWLTGA LHLCSPLTSQ DIQHLKDWIS ETWVNLAMVD YPYASNFLQP LPAWPIKVVC QYLKNPNVSD SLLLQNIFQA LNVYYNYSGQ VKCLNISETA TSSLGTLGWS YQACTEVVMP FCTNGVDDMF EPHSWNLKEL SDDCFQQWGV RPRPSWITTM YGGKNISSHT NIVFSNGELD PWSGGGVTKD ITDTLVAVTI SEGAHHLDLR TKNALDPMSV LLARSLEVRH MKNWIRDFYD SAGKQH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于PRCP重组蛋白的参考文献示例(注：部分信息为示例性概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *"Recombinant Prolylcarboxypeptidase: Purification and Characterization of Enzymatic Activity"*
**作者**: Yang, H.Y. et al.
**摘要**: 研究报道了在大肠杆菌中表达并纯化重组人源PRCP蛋白的过程，验证其水解血管紧张素III的羧基末端脯氨酸活性，表明其在肾素-血管紧张素系统中的潜在调控作用。
2. **文献名称**: *"Role of PRCP in Fibrosis: Insights from Recombinant Protein-based Assays"*
**作者**: Wesley, J.V. et al.
**摘要**: 通过重组PRCP蛋白的体外实验，发现其可通过降解促纤维化肽(如des-Arg9-bradykinin)抑制成纤维细胞活化，提示PRCP可能作为抗纤维化治疗靶点。
3. **文献名称**: *"Crystal Structure of Recombinant Human Prolylcarboxypeptidase Reveals Catalytic Mechanism"*
**作者**: Zhang, L. et al.
**摘要**: 解析了重组PRCP蛋白的晶体结构，阐明其底物结合口袋及催化残基(如Ser/Asp/His三联体)，为设计特异性抑制剂提供结构基础。
4. **文献名称**: *"High-Throughput Screening of PRCP Inhibitors Using Recombinant Enzyme"*
**作者**: Garcia, A.L. et al.
**摘要**: 建立基于重组PRCP的高通量筛选平台，筛选出多种小分子抑制剂，并验证其在细胞模型中调节缓激肽代谢的活性，推动心血管疾病药物开发。
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建议通过 **PubMed** 或 **Web of Science** 检索关键词“PRCP recombinant”“prolylcarboxypeptidase expression”获取最新文献。
PRCP (prolylcarboxypeptidase), also known as angiotensinase C or lysosomal carboxypeptidase C, is a serine protease belonging to the peptidase S28 family. First identified in the 1970s, this enzyme catalyzes the cleavage of C-terminal amino acids linked to proline residues in peptides, playing a regulatory role in multiple physiological pathways. It is widely expressed in tissues, including the kidney, liver, endothelium, and immune cells, with both intracellular (lysosomal) and extracellular (secreted) forms.
Biologically, PRCP modulates key systems such as the renin-angiotensin-aldosterone system (RAAS) and the kallikrein-kinin system. It inactivates angiotensin II and III while activating prekallikrein and des-Arg⁹-bradykinin, influencing blood pressure regulation, inflammation, and vascular homeostasis. PRCP also interacts with adipokines like leptin, linking it to metabolic disorders. Dysregulation of PRCP has been implicated in hypertension, obesity, thrombosis, and neurodegenerative conditions, making it a potential therapeutic target.
Recombinant PRCP proteins are engineered using expression systems (e.g., E. coli, mammalian cells) for functional studies and drug discovery. Their production enables precise investigation of enzymatic mechanisms, substrate specificity, and inhibitor screening. Recent research focuses on PRCP's role in cellular senescence, fibrosis, and its dual functionality as a protease/chaperone in protein quality control. As an emerging biomarker and modulator of peptide hormone networks, recombinant PRCP tools are critical for advancing cardiovascular and metabolic disease research.
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