| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KARS |
| Uniprot No | Q15046 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 63-597aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSGVGPEEESVDPNQYYKIRSQAIHQLKV NGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASG GKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPG KTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDF VRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNE LDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCE FYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTP PFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRT TARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELF VMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEY GLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLE STTVGTSV |
| 预测分子量 | 64 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KARS(赖氨酰-tRNA合成酶)重组蛋白研究的模拟参考文献示例(仅供参考，建议通过学术数据库核实具体文献)：
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1. **文献名称**：*Expression and Functional Analysis of Recombinant Human KARS in Cancer Cell Proliferation*
**作者**：Park, S. et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组人KARS蛋白，发现其在多种癌细胞中高表达，并通过体外实验证明其通过调控MAPK信号通路促进肿瘤细胞增殖。
2. **文献名称**：*Crystal Structure of Recombinant KARS Reveals a Potential Drug Target*
**作者**：Lee, J. & Kim, H.
**摘要**：利用X射线晶体学解析了重组KARS蛋白的三维结构，揭示了其ATP结合位点的关键氨基酸残基，为开发针对感染性疾病的小分子抑制剂提供了结构基础。
3. **文献名称**：*Enzymatic Characterization of Recombinant Lysyl-tRNA Synthetase from Mycobacterium tuberculosis*
**作者**：Gupta, R. et al.
**摘要**：通过重组技术表达了结核分枝杆菌KARS蛋白，并测定其酶动力学参数，发现其与宿主同源酶活性差异显著，提示其作为抗结核药物靶点的潜力。
4. **文献名称**：*Role of KARS in Neurodegeneration: Insights from Recombinant Protein Models*
**作者**：Zhang, Y. et al.
**摘要**：通过重组KARS蛋白的体外实验，发现其错误折叠可激活未折叠蛋白反应(UPR)，可能参与阿尔茨海默病中tau蛋白病理的调控机制。
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建议通过PubMed或Google Scholar搜索关键词“recombinant KARS protein”、“Lysyl-tRNA synthetase expression”获取最新文献。
**Background of KARS Recombinant Protein**
KARS (Lysyl-tRNA Synthetase) is an essential enzyme in the aminoacylation process during protein synthesis. It catalyzes the attachment of lysine to its cognate tRNA, ensuring the accurate translation of genetic code into functional proteins. As a member of the aminoacyl-tRNA synthetase (ARS) family, KARS plays a dual role: beyond its canonical function in translation, it is implicated in diverse cellular processes, including transcriptional regulation, immune signaling, and apoptosis.
Recombinant KARS protein is produced via genetic engineering, typically using expression systems like *E. coli* or mammalian cells. The process involves cloning the KARS gene into a plasmid, followed by transfection, expression, and purification to obtain a highly pure, functional protein. This recombinant form retains enzymatic activity and structural integrity, making it a valuable tool for biochemical and structural studies.
Research on KARS has gained momentum due to its association with human diseases. Mutations in KARS are linked to neuropathies, hearing loss, and leukodystrophies, while its overexpression or aberrant activity is observed in cancers and autoimmune disorders. Recombinant KARS enables mechanistic studies to dissect these disease connections and screen for therapeutic compounds targeting its enzymatic or non-canonical functions.
In biotechnology, recombinant KARS is utilized in enzyme engineering and synthetic biology to optimize translation machinery or develop novel biosensors. Its structural flexibility and conserved catalytic domains also make it a model for studying ARS evolution and substrate specificity.
Overall, KARS recombinant protein bridges fundamental research and therapeutic innovation, offering insights into both cellular biology and disease mechanisms.
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