Mouse Monoclonal RAB5B Antibody

The RAB5B antibody is a crucial tool for studying the RAB5B protein, a member of the RAB GTPase family involved in regulating intracellular membrane trafficking. RAB5B localizes to early endosomes and plays a key role in endocytosis, early endosome fusion, and signal transduction by cycling between active GTP-bound and inactive GDP-bound states. Dysregulation of RAB5B has been linked to diseases such as cancer, neurodegenerative disorders, and immune dysfunction. Researchers use RAB5B antibodies (polyclonal or monoclonal) to detect and quantify the protein’s expression, localization, and interactions in techniques like Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC). These antibodies are often validated for specificity through knockdown controls, peptide blocking assays, or cross-reactivity checks with related isoforms (e.g., RAB5A, RAB5C). Commercial suppliers (e.g., CST, Abcam, Sigma-Aldrich) provide RAB5B antibodies with species-specific reactivity (human, mouse, rat), though cross-species applications may require validation. Challenges include distinguishing RAB5B from homologous proteins and ensuring consistent performance across experimental conditions. Recent studies highlight its role in cancer metastasis and autophagy, making RAB5B antibodies vital for exploring therapeutic targets. Proper controls (e.g., knockout cell lines) are essential to confirm antibody reliability in experimental setups.