| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BIN2 |
| Uniprot No | Q9UBW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-244aa |
| 氨基酸序列 | MAEGKAGGAAGLFAKQVQKKFSRAQEKVLQKLGKAVETKDERFEQSASNF YQQQAEGHKLYKDLKNFLSAVKVMHESSKRVSETLQEIYSSEWDGHEELK AIVWNNDLLWEDYEEKLADQAVRTMEIYVAQFSEIKERIAKRGRKLVDYD SARHHLEAVQNAKKKDEAKTAKAEEEFNKAQTVFEDLNQELLEELPILYN SRIGCYVTIFQNISNLRDVFYREMSKLNHNLYEVMSKLEKQHSN |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BIN2重组蛋白的3篇示例文献(内容为假设性概括，供参考格式)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant BIN2 Kinase in Arabidopsis*
**作者**：Li et al.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化重组BIN2蛋白，通过体外激酶实验证实其可磷酸化下游转录因子BZR1.揭示了BIN2在油菜素内酯信号通路中的负调控机制。
2. **文献名称**：*Structural Insights into BIN2 Kinase Activity via Recombinant Protein Crystallography*
**作者**：Wang et al.
**摘要**：通过昆虫细胞表达系统获得高纯度重组BIN2蛋白，解析其晶体结构，发现ATP结合口袋的关键氨基酸残基，为设计BIN2特异性抑制剂提供了结构基础。
3. **文献名称**：*Recombinant BIN2 Interaction with PP2A Phosphatase Modulates Brassinosteroid Signaling*
**作者**：Zhang et al.
**摘要**：利用重组BIN2和PP2A蛋白进行体外结合实验，证明两者直接互作并协同调控BZR1的磷酸化状态，阐明了BIN2与磷酸酶动态平衡在信号传导中的作用。
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**说明**：以上文献为示例，实际研究中建议通过数据库(如PubMed、Google Scholar)以关键词“BIN2 recombinant protein”“BIN2 kinase structure”检索最新文献。研究多聚焦于BIN2的激酶活性、结构解析及其在油菜素内酯信号通路中的调控机制。
**Background of BIN2 Recombinant Protein**
BIN2 (Brassinosteroid-Insensitive 2) is a serine/threonine protein kinase belonging to the glycogen synthase kinase 3 (GSK3) family, first identified in *Arabidopsis thaliana*. It plays a critical role in brassinosteroid (BR) signaling, a pathway essential for plant growth, development, and stress responses. BIN2 acts as a negative regulator of BR signaling by phosphorylating transcription factors such as BZR1 and BES1. promoting their degradation or cytoplasmic retention, thereby suppressing BR-responsive gene expression.
Recombinant BIN2 proteins are engineered for in vitro studies to elucidate its structure, enzymatic activity, and interaction with substrates or inhibitors. These proteins are typically produced in heterologous systems like *E. coli* or yeast, enabling high-yield purification. The recombinant form retains kinase activity, allowing researchers to investigate phosphorylation mechanisms, screen for modulators, or study cross-talk with other signaling pathways (e.g., light or stress responses).
BIN2's functional conservation across plant species and structural similarity to mammalian GSK3 kinases also make it a model for studying kinase evolution and regulatory mechanisms. Its recombinant versions are pivotal in agricultural biotechnology for engineering crops with improved stress tolerance or growth traits. Additionally, BIN2 studies contribute to understanding human diseases linked to GSK3 dysregulation, such as cancer or neurodegenerative disorders, by providing comparative insights into kinase regulation.
In summary, BIN2 recombinant protein serves as a versatile tool for dissecting BR signaling, exploring kinase biology, and bridging plant and biomedical research.
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