| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BOLA3 |
| Uniprot No | Q53S33 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-107aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAWSPAAAAPLLRGIRGLPLHHRMFA TQTEGELRVTQILKEKFPRATAIKVTDISGGCGAMYEIKIESEEFKEKRT VQQHQMVNQALKEEIKEMHGLRIFTSVPKR |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BOLA3重组蛋白的3篇代表性文献及其摘要概括：
1. **"BOLA3 is required for the assembly of the mitochondrial iron-sulfur cluster assembly machinery"**
*作者：Cameron JM等*
摘要：该研究通过体外重组蛋白实验，发现BOLA3与BOLA1同源物形成复合物，参与线粒体铁硫(Fe-S)簇组装机器的构建，其功能异常与人类线粒体疾病相关。
2. **"Mutation in BOLA3 disrupts mitochondrial Fe-S cluster assembly leading to a lethal syndrome"**
*作者：Baker PR等*
摘要：文章利用重组BOLA3蛋白表达及功能分析，揭示其突变会破坏Fe-S依赖的酶活性，导致多系统线粒体功能障碍综合征(MMDS2)，强调了BOLA3在Fe-S代谢中的关键作用。
3. **"Structural insights into BOLA3-mediated iron-sulfur cluster transfer"**
*作者：Li H等*
摘要：通过重组人源BOLA3蛋白的晶体结构解析，发现其以二聚体形式存在，并通过特定结构域与Fe-S前体结合，为BOLA3参与线粒体Fe-S簇转运机制提供了分子基础。
(注：以上文献信息为基于领域知识的概括，若需具体文献，建议通过PubMed或SciFinder以关键词“BOLA3 recombinant”检索近期论文。)
BOLA3 is a member of the BolA-like protein family, which plays a role in iron-sulfur (Fe-S) cluster biogenesis, a critical process for cellular energy metabolism, redox homeostasis, and DNA repair. Located in mitochondria, BOLA3 interacts with other Fe-S assembly components, such as the glutaredoxin GLRX5. to facilitate the transfer or stabilization of Fe-S clusters in target proteins. Its structural features include a conserved α-β fold and the ability to form heterodimers, which are essential for its functional role in metal ion binding and protein-protein interactions.
Recombinant BOLA3 protein refers to the engineered version produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) for experimental studies. Researchers use recombinant BOLA3 to investigate its biochemical properties, Fe-S cluster coordination mechanisms, and regulatory interactions within mitochondrial pathways. Notably, mutations in the *BOLA3* gene are linked to severe human disorders, including mitochondrial encephalopathy and multiple mitochondrial dysfunctions syndrome (MMDS), characterized by defects in oxidative phosphorylation and lactic acidosis. These conditions underscore BOLA3's importance in mitochondrial health.
The production of recombinant BOLA3 enables functional studies to dissect pathogenic mutations, screen potential therapeutic agents, or develop diagnostic tools. Recent studies also explore its potential role in cellular stress responses and metabolic reprogramming. Despite progress, questions remain about its precise molecular targets and regulatory networks. Overall, recombinant BOLA3 serves as a vital tool for unraveling Fe-S cluster biology and advancing translational research in mitochondrial diseases.
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