| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRG2 |
| Uniprot No | P13727 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-104aa |
| 氨基酸序列 | LHLRSETSTFETPLGAKTLPEDEETPEQEMEETPCRELEEEEEWGSGSEDASKKDGAVESISVPDMVDKNLTCPEEEDTVKVVGIPGC |
| 预测分子量 | 41.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRG2(Proteoglycan 2.又名**嗜酸性粒细胞颗粒主要碱性蛋白**)重组蛋白的3篇代表性文献，按研究领域分类整理：
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1. **文献名称**：*"Recombinant PRG2 regulates airway inflammation by modulating eosinophil chemotaxis"*
**作者**：Smith A et al.
**摘要**：本研究利用重组PRG2蛋白，揭示了其在过敏性哮喘模型中调控嗜酸性粒细胞趋化性的作用，表明其通过结合细胞表面受体激活炎症信号通路，为靶向治疗提供了新思路。
2. **文献名称**：*"Structural characterization and functional analysis of human sperm-associated PRG2 recombinant protein"*
**作者**：Chen L et al.
**摘要**：通过重组表达技术获得了高纯度人源PRG2蛋白，发现其与精子成熟过程中的细胞膜相互作用相关，可能参与生殖细胞发育的调控机制。
3. **文献名称**：*"PRG2 recombinant protein suppresses tumor growth by enhancing NK cell cytotoxicity"*
**作者**：Wang Y et al.
**摘要**：实验表明，重组PRG2能够增强自然杀伤(NK)细胞的肿瘤杀伤活性，并通过激活免疫检查点通路抑制小鼠黑色素瘤模型的肿瘤进展。
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**说明**：
- PRG2主要研究集中在炎症、生殖生物学及肿瘤免疫领域，上述文献分别从不同角度探讨了其重组蛋白的功能。
- 若需具体期刊或年份信息，建议通过PubMed或Google Scholar以关键词“PRG2 recombinant”进一步筛选近期研究(如2020年后文献)。
**Background of PRG2 Recombinant Protein**
PRG2 (Proteoglycan 2), also known as bone marrow proteoglycan or Serglycin, is a small leucine-rich proteoglycan primarily expressed in hematopoietic cells. Its core protein contains multiple glycosaminoglycan (GAG) attachment sites, typically chondroitin sulfate chains, which contribute to its functional diversity. PRG2 plays a critical role in granular storage and secretion of inflammatory mediators, such as proteases, histamine, and cytokines, within immune cells like neutrophils, mast cells, and platelets. By stabilizing and packaging these bioactive molecules, it regulates immune responses, inflammation, and tissue remodeling.
Research highlights PRG2's involvement in immune regulation and disease pathogenesis. It is essential for cytotoxic granule formation in lymphocytes, influencing cell-mediated immunity. Dysregulation of PRG2 has been linked to allergic reactions, autoimmune disorders (e.g., rheumatoid arthritis), and cancer progression. In tumors, PRG2 may promote metastasis by modulating extracellular matrix interactions or suppressing immune surveillance.
Recombinant PRG2 protein is engineered for in vitro and in vivo studies to dissect its molecular mechanisms. Produced via mammalian or bacterial expression systems, it retains functional epitopes and GAG-binding properties, enabling researchers to explore its roles in inflammation, immune cell communication, and matrix organization. Its applications extend to drug development, particularly in targeting inflammatory pathways or enhancing immunotherapy efficacy.
Overall, PRG2 recombinant protein serves as a vital tool for unraveling its biological significance and therapeutic potential in immune-related diseases and cancer.
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