| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETS1 |
| Uniprot No | Q96AC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-272aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMKAAVDLKPTLTIIKTEKVDLELFPSPDME CADVPLLTPSSKEMMSQALKATFSGFTKEQQRLGIPKDPRQWTETHVRDW VMWAVNEFSLKGVDFQKFCMNGAALCALGKDCFLELAPDFVGDILWEHLE ILQKEDVKPYQVNGVNPAYPESRYTSDYFISYGIEHAQCVPPSEFSEPSF ITESYQTLHPISSEELLSLKYENDYPSVILRDPLQTDTLQNDYFAIKQEV VTPDNMCMGRTSRGKLGGQDSFESIESYDSCGQEMGKEEKQT |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于ETS1重组蛋白的参考文献及简要摘要：
1. **文献名称**：*"Expression and purification of recombinant human ETS1 transcription factor in Escherichia coli"*
**作者**：Smith J, et al.
**摘要**：该研究报道了通过原核表达系统(大肠杆菌)成功表达并纯化重组人ETS1蛋白，验证了其DNA结合活性及在体外转录调控中的应用。
2. **文献名称**：*"Structural and functional analysis of ETS1 phosphorylation sites using recombinant protein mutants"*
**作者**：Johnson R, et al.
**摘要**：通过构建ETS1重组蛋白的磷酸化位点突变体，结合质谱和凝胶迁移实验(EMSA)，揭示了特定磷酸化修饰对ETS1与靶DNA结合能力的影响。
3. **文献名称**：*"Recombinant ETS1 protein regulates oncogenic signaling in breast cancer cells"*
**作者**：Lee S, et al.
**摘要**：研究利用重组ETS1蛋白处理乳腺癌细胞，发现其通过调控MMP-9和VEGF等靶基因表达，影响肿瘤细胞的侵袭和血管生成能力。
4. **文献名称**：*"Crystal structure of the ETS1 dimerization domain and its functional implications"*
**作者**：Brown K, et al.
**摘要**：通过X射线晶体学解析重组ETS1蛋白的二聚化结构域，揭示了其通过疏水相互作用形成二聚体的机制，并证明二聚化对转录激活功能至关重要。
(注：上述文献为虚拟示例，实际引用时需以真实发表的论文为准。)
**Background of ETS1 Recombinant Protein**
ETS1 (E26 transformation-specific 1) is a member of the ETS family of transcription factors, characterized by a conserved DNA-binding domain (ETS domain) that binds to purine-rich sequences in gene promoters. Initially identified in avian erythroblastosis virus, ETS1 plays pivotal roles in regulating gene expression involved in cellular processes such as immune regulation, angiogenesis, apoptosis, and cancer progression. It is expressed in various tissues, including lymphocytes, endothelial cells, and certain cancer cells, where it modulates target genes like *MMP9*, *VEGF*, and *BCL2*.
The recombinant ETS1 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems, to ensure proper post-translational modifications (e.g., phosphorylation) critical for its activity. Purification methods like affinity chromatography yield high-purity protein for research applications. Recombinant ETS1 retains the ability to bind DNA and regulate transcription, making it a valuable tool for studying mechanisms of gene regulation, protein-DNA interactions (e.g., EMSA, ChIP), and signaling pathways in diseases.
In cancer research, ETS1 overexpression is linked to tumor invasion and metastasis, driving studies on its oncogenic potential. In immunology, it regulates T-cell development and cytokine production, implicating it in autoimmune diseases. Recombinant ETS1 variants (e.g., mutants or truncated forms) further enable dissection of functional domains or disease-associated mutations.
Overall, ETS1 recombinant protein serves as a critical reagent for dissecting transcriptional networks and developing therapeutic strategies targeting ETS1-driven pathologies.
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