| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD72 |
| Uniprot No | P21854 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 117-359aa |
| 氨基酸序列 | RYLQVSQQLQ QTNRVLEVTN SSLRQQLRLK ITQLGQSAE DLQGSRRELA QSQEALQVEQ RAHQAAEGQL QACQADRQKT KETLQSEEQQ RRALEQKLSN MENRLKPFFT CGSADTCCPS GWIMHQKSCF YISLTSKNWQ ESQKQCETLS SKLATFSEIY PQSHSYYFLN SLLPNGGSGN SYWTGLSSNK DWKLTDDTQR TRTYAQSSKC NKVHKTWSWW TLESESCRSS LPYICEMTAF RFPD Fc(EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK) |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD72重组蛋白的3篇示例参考文献(内容为模拟概括，仅供参考)：
1. **《CD72重组蛋白的制备及其在B细胞信号转导中的功能研究》**
- 作者：T. Kurosaki, Y. Maeda
- 摘要：本研究通过大肠杆菌表达系统成功纯化CD72重组蛋白，并发现其通过ITIM结构域介导抑制性信号，调控B细胞受体(BCR)的激活阈值，为自身免疫疾病机制提供新见解。
2. **《CD72与CD100的相互作用调控免疫耐受的分子机制》**
- 作者：L. Li, J. Wu
- 摘要：利用CD72重组蛋白进行体外结合实验，证实CD72作为CD100的配体，通过解离抑制性复合物促进B细胞活化，揭示了其在维持免疫稳态中的双向调节作用。
3. **《CD72重组蛋白在系统性红斑狼疮模型中的治疗潜力》**
- 作者：M. Sato, R. Nakauchi
- 摘要：动物实验表明，可溶性CD72重组蛋白能竞争性阻断病理性的B细胞过度活化，显著减轻狼疮模型小鼠的肾脏损伤，提示其作为免疫调节剂的潜在应用价值。
**提示**：以上为模拟示例，实际文献需通过PubMed、Web of Science或Google Scholar检索关键词“CD72 recombinant protein”获取。建议筛选近年(如2015年后)研究以反映最新进展。
CD72. also known as CD72 antigen or Lyb-2. is a type II transmembrane glycoprotein belonging to the C-type lectin receptor family. It is primarily expressed on B lymphocytes and antigen-presenting cells (APCs), playing a critical role in modulating immune responses. As a co-receptor of the B-cell receptor (BCR), CD72 regulates B-cell activation, differentiation, and tolerance by transmitting both inhibitory and activating signals. Its extracellular C-type lectin-like domain interacts with ligands such as CD100 (SEMA4D), while its cytoplasmic tail contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit phosphatases like SHP-1 to dampen BCR signaling. This dual functionality positions CD72 as a key checkpoint in balancing immune activation and suppression.
Dysregulation of CD72 has been implicated in autoimmune diseases (e.g., lupus), B-cell malignancies, and chronic inflammatory conditions. Its expression patterns and signaling pathways make it a potential therapeutic target. Recombinant CD72 proteins, typically produced in mammalian or insect expression systems, retain the functional domains required for ligand binding and signal modulation. These engineered proteins are widely used in immunological research to study B-cell biology, protein-protein interactions, and signal transduction mechanisms. Additionally, they serve as critical tools for developing antibody-based therapies, diagnostic assays, and vaccine adjuvants. Recent studies also explore CD72's role in tumor microenvironments and its potential as a biomarker for certain cancers, further expanding its relevance in both basic research and clinical applications.
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