| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SELENOI |
| Uniprot No | Q9C0D9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-397aa |
| 氨基酸序列 | MAGYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSTGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWSPSDILELHPRVFYFMVGTAFANSTCQLIVCQMSSTRCPTLNWLLVPLFLVVLVVNLGVASYVESILLYTLTTAFTLAHIHYGVRVVKQLSSHFQIYPFSLRKPNSDULGMEEKNIGL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SELENOI(EPT1)重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*"Identification and characterization of human ethanolaminephosphotransferase1 (SELENOI)"*
**作者**：Horibata Y, et al.
**摘要**：该研究首次成功克隆并表达人源SELENOI重组蛋白，证实其催化乙醇胺磷脂生物合成的关键功能，并揭示其在内质网中的定位及对细胞膜结构的影响。
2. **文献名称**：*"SELENOI deficiency disrupts lipid metabolism in CD4+ T cells"*
**作者**：Alonso F, et al.
**摘要**：通过重组蛋白功能回补实验，发现SELENOI缺失导致T细胞中磷脂酰乙醇胺合成受阻，引发内质网应激并抑制细胞增殖，揭示了其在免疫调控中的代谢作用。
3. **文献名称**：*"A selenoprotein-like enzyme essential for neuronal development"*
**作者**：Shchedrina VA, et al.
**摘要**：利用重组SELENOI蛋白进行酶活分析，发现其结构中的硒代半胱氨酸残基对催化活性至关重要，并证明其功能缺陷会导致神经细胞发育异常及运动失调表型。
注：若需具体文献年份或期刊信息，可补充说明进一步筛选。
SELENOI, also known as Selenoprotein I (SELENOI), is a member of the selenoprotein family characterized by the presence of selenocysteine (Sec), a rare amino acid encoded by the UGA codon. This protein is evolutionarily conserved and plays a critical role in phospholipid biosynthesis, specifically in the Kennedy pathway. SELENOI functions as an ethanolamine phosphotransferase (EPT1), catalyzing the final step in the synthesis of phosphatidylethanolamine (PE), a major phospholipid essential for membrane structure, vesicle trafficking, and cellular signaling. Its enzymatic activity requires the incorporation of selenium, which underscores the importance of dietary selenium in maintaining its function.
The gene encoding SELENOI, *EPT1*, is located on human chromosome 2 and is ubiquitously expressed, with higher levels observed in metabolically active tissues like the liver, brain, and kidneys. Dysregulation of SELENOI has been linked to neurological disorders, immune dysfunction, and cancer, highlighting its physiological significance. For instance, impaired PE synthesis due to SELENOI deficiency can disrupt neuronal membrane integrity, contributing to neurodegeneration.
Recombinant SELENOI protein is engineered using heterologous expression systems (e.g., mammalian or bacterial cells) to study its structure, enzymatic mechanisms, and interactions. This recombinant tool enables researchers to explore its role in lipid metabolism, cellular homeostasis, and disease pathogenesis. Additionally, it aids in screening potential therapeutic agents targeting SELENOI-related pathways. Studies using recombinant SELENOI have also clarified how selenium availability modulates its activity, linking environmental factors to metabolic health. Overall, SELENOI recombinant protein serves as a vital resource for advancing our understanding of selenoprotein biology and lipid-driven pathologies.
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