| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BOLA1 |
| Uniprot No | Q9Y3E2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-137aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSQGSAGSG AIGPVEAAIR TKLEEALSPE VLELRNESGG HAVPPGSETH FRVAVVSSRF EGLSPLQRHR LVHAALAEEL GGPVHALAIQ ARTPAQWREN SQLDTSPPCL GGNKKTLGTP |
| 预测分子量 | 14 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BOLA1重组蛋白的示例参考文献(注：以下内容为虚构示例，建议通过学术数据库查询真实文献)：
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1. **文献名称**: *"Recombinant BOLA1 Expression and Its Role in Iron-Sulfur Cluster Biogenesis"*
**作者**: Smith, J. et al.
**摘要**: 本研究在大肠杆菌中成功表达了重组BOLA1蛋白，并验证其与线粒体铁硫簇组装蛋白(如GLRX5)的相互作用。实验表明，BOLA1可能通过调节谷胱甘肽依赖的途径参与细胞氧化还原平衡。
2. **文献名称**: *"Structural Characterization of Human BOLA1 Using Recombinant Protein Technology"*
**作者**: Zhang, L. et al.
**摘要**: 通过X射线晶体学解析了重组人源BOLA1蛋白的三维结构，揭示了其独特的α-螺旋折叠模式及潜在的金属结合位点，为研究其分子功能提供了结构基础。
3. **文献名称**: *"BOLA1 Recombinant Protein Enhances Cellular Antioxidant Defense in vitro"*
**作者**: Tanaka, K. et al.
**摘要**: 利用HEK293细胞系表达的重组BOLA1蛋白，发现其过表达可显著降低活性氧(ROS)水平，提示BOLA1在抗氧化应激中可能发挥保护作用。
4. **文献名称**: *"Cloning, Expression, and Purification of BOLA1 for Functional Proteomic Studies"*
**作者**: Chen, W. et al.
**摘要**: 开发了一种高效的可溶性BOLA1重组蛋白表达及纯化方案，并通过质谱分析鉴定了其互作蛋白网络，揭示了其在线粒体代谢中的潜在新靶点。
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建议通过 **PubMed**、**Web of Science** 或 **Google Scholar** 搜索关键词 "BOLA1 recombinant protein" 或 "BOLA1 expression" 获取真实文献。
**Background of BOLA1 Recombinant Protein**
BOLA1 (BolA Family Member 1) is a conserved eukaryotic protein belonging to the BolA-like family, which is implicated in cellular iron homeostasis and redox regulation. Initially identified in bacteria, BolA proteins are evolutionarily linked to stress response pathways. In humans, BOLA1 localizes to mitochondria and interacts with components of the iron-sulfur cluster (ISC) biogenesis machinery, particularly glutaredoxin-5 (Grx5), suggesting its role in Fe-S cluster assembly or trafficking. These clusters are essential cofactors for enzymes involved in critical processes, including electron transport, DNA repair, and metabolic regulation.
BOLA1 is structurally characterized by a conserved BolA domain, facilitating protein-protein interactions. Studies suggest it forms heterocomplexes with monothiol glutaredoxins, acting as a redox-sensitive regulator to maintain mitochondrial iron balance and mitigate oxidative stress. Dysregulation of BOLA1 has been associated with mitochondrial dysfunction, oxidative damage, and pathologies such as neurodegenerative disorders and cancer, though its precise mechanisms remain under investigation.
Recombinant BOLA1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enabling studies on its biochemical properties, structural dynamics, and interactions. Its recombinant form is vital for *in vitro* assays, structural biology (e.g., crystallography), and exploring therapeutic strategies targeting iron metabolism or oxidative stress-related diseases. Research on BOLA1 continues to unravel its dual role in cellular redox regulation and iron management, positioning it as a potential biomarker or therapeutic target in mitochondrial disorders.
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