| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATG4C |
| Uniprot No | Q96DT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-458aa |
| 氨基酸序列 | MEATGTDEVD KLKTKFISAW NNMKYSWVLK TKTYFSRNSP VLLLGKCYHF KYEDEDKTLP AESGCTIEDH VIAGNVEEFR KDFISRIWLT YREEFPQIEG SALTTDCGWG CTLRTGQMLL AQGLILHFLG RAWTWPDALN IENSDSESWT SHTVKKFTAS FEASLSGERE FKTPTISLKE TIGKYSDDHE MRNEVYHRKI ISWFGDSPLA LFGLHQLIEY GKKSGKKAGD WYGPAVVAHI LRKAVEEARH PDLQGITIYV AQDCTVYNSD VIDKQSASMT SDNADDKAVI ILVPVRLGGE RTNTDYLEFV KGILSLEYCV GIIGGKPKQS YYFAGFQDDS LIYMDPHYCQ SFVDVSIKDF PLETFHCPSP KKMSFRKMDP SCTIGFYCRN VQDFKRASEE ITKMLKFSSK EKYPLFTFVN GHSRDYDFTS TTTNEEDLFS EDEKKQLKRF STEEFVLL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATG4C重组蛋白的3篇参考文献的简要概括：
1. **文献名称**：*"Characterization of recombinant human ATG4C as a cysteine protease involved in autophagy"*
**作者**：Li M. et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组人源ATG4C蛋白，证实其具有切割LC3前体的酶活性，并发现其活性受氧化应激调控，为自噬机制的分子基础提供了新见解。
2. **文献名称**：*"Substrate specificity of the ATG4 family members: Comparative analysis using recombinant ATG4 proteins"*
**作者**：Maruyama T. et al.
**摘要**：通过比较重组表达的ATG4A-D蛋白，研究发现ATG4C对LC3家族底物的选择性不同于其他成员，且其酶切效率在特定自噬刺激条件下显著增强，揭示了家族成员的功能分化。
3. **文献名称**：*"ATG4C regulates mitochondrial function and oxidative stress through cleavage of mitophagy adapters"*
**作者**：Kwon Y. et al.
**摘要**：利用重组ATG4C蛋白进行体外实验，发现其不仅能处理LC3.还可切割线粒体自噬衔接蛋白，提示ATG4C在维持线粒体稳态及抗氧化应激中的双重作用，可能与神经退行性疾病相关。
(注：上述文献为示例性概括，实际引用需以真实发表的论文为准。)
ATG4C, a member of the ATG4 cysteine protease family, plays a critical role in autophagy—a conserved cellular recycling process. This protein specifically processes LC3 (microtubule-associated protein 1 light chain 3), a key autophagy-related protein, by cleaving its C-terminal arginine to expose a glycine residue. This step is essential for LC3 lipidation (conjugation to phosphatidylethanolamine), which drives autophagosome membrane expansion and cargo sequestration. ATG4C also mediates LC3 delipidation to recycle it from autophagosomal membranes post-fusion with lysosomes.
Structurally, ATG4C contains a catalytic cysteine residue within its papain-like protease domain, enabling substrate recognition and cleavage. While all four ATG4 homologs (ATG4A-D) share overlapping functions in LC3 processing, ATG4C exhibits unique tissue-specific expression patterns and regulatory features. Studies suggest its activity is modulated by post-translational modifications, including oxidation and phosphorylation, which may fine-tune autophagy under stress conditions.
Recombinant ATG4C protein, produced via bacterial or mammalian expression systems, is widely used to study autophagy mechanisms in vitro. Its applications include enzymatic activity assays, substrate specificity profiling, and inhibitor screening. Research has linked ATG4C dysregulation to cancer progression, neurodegenerative diseases, and metabolic disorders, positioning it as a potential therapeutic target. Knockout mouse models reveal compensatory mechanisms among ATG4 homologs, underscoring functional redundancy in vivo. Recent studies also explore its non-autophagic roles in processes like apoptosis and immune response regulation, broadening its biological significance.
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