| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SWSAP1 |
| Uniprot No | Q6NVH7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-229aa |
| 氨基酸序列 | MNHKVHHHHHHMPAAGPPLLLLGTPGSGKTALLFAAALEAAGEGQGPVLF LTRRPLQSMPRGTGTTLDPMRLQKIRFQYPPSTRELFRLLCSAHEAPGPA PSLLLLDGLEEYLAEDPEPQEAAYLIALLLDTAAHFSHRLGPGRDCGLMV ALQTQEEAGSGDVLHLALLQRYFPAQCWLQPDAPGPGEHGLRACLEPGGL GPRTEWWVTFRSDGEMMIAPWPTQAGDPSSGKGSSSGGQP |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SWSAP1重组蛋白的3条示例参考文献及摘要概括：
1. **"SWSAP1 is essential for BRCA1-mediated homologous recombination in human cells"**
- **作者**: Smith J, et al.
- **摘要**: 本研究揭示SWSAP1在BRCA1依赖的同源重组(HR)修复中的作用，通过调控RAD51丝的形成促进DNA双链断裂修复，缺失SWSAP1导致基因组不稳定性和化疗敏感性增加。
2. **"SWSAP1 interacts with RAD51 and DMC1 to promote meiotic recombination in mammals"**
- **作者**: Tanaka K, et al.
- **摘要**: 实验证明SWSAP1在小鼠减数分裂中与重组酶RAD51/DMC1直接互作，协助同源染色体配对与交叉形成，敲除SWSAP1引发配子发生缺陷和不育表型。
3. **"Structural insights into SWSAP1’s role in homology-directed DNA repair"**
- **作者**: Chen L, et al.
- **摘要**: 通过冷冻电镜解析SWSAP1蛋白复合体结构，发现其N端结构域对结合单链DNA和招募RAD51至关重要，阐明其在同源重组中的分子机制。
(注：以上文献为示例，实际引用需根据具体研究检索PubMed等数据库。)
SWSAP1 (Swi5-dependent homologous recombination repair protein 1) is a conserved eukaryotic protein involved in DNA repair and homologous recombination processes, particularly during meiosis. It belongs to the SWS1 protein family, which shares homology with the archaeal DNA repair protein RadA/Sms. SWSAP1 is structurally characterized by a conserved SWS1-associated protein (SAP) domain, facilitating interactions with other DNA repair proteins like RAD51 and DMC1. These interactions are critical for stabilizing recombination intermediates and ensuring accurate chromosome segregation.
In mammals, SWSAP1 is essential for gametogenesis and fertility. Studies in mice have shown that SWSAP1 knockout models exhibit severe defects in meiotic progression, leading to infertility due to impaired double-strand break (DSB) repair and homologous chromosome pairing. The protein is thought to act as a co-factor for RAD51 and DMC1 recombinases, promoting strand invasion and homology search during meiotic recombination. Its role extends to somatic cells, where it contributes to mitotic DNA repair, though its absence primarily manifests in reproductive tissues.
SWSAP1's evolutionary conservation across species—from yeast (where homologs like SWI5 participate in recombination) to humans—underscores its fundamental role in genome stability. Dysregulation of SWSAP1 has been tentatively linked to reproductive disorders and cancer susceptibility, though direct clinical correlations remain under investigation. Recombinant SWSAP1 proteins are often expressed in bacterial or insect cell systems for biochemical assays, structural studies, and interaction mapping. These tools help elucidate its molecular mechanisms, such as ATPase activity or DNA-binding properties, advancing our understanding of homologous recombination pathways and their implications in disease and fertility.
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