Rabbit Polyclonal Zebrafishak2 Antibody

+ +

•  

   
     All lanes : Anti-Zebrafish ak2 Antibody (Center) at 1:2000 dilution Lane 1: Zebrafish lysates Lane 2: ZF4 whole cell lysates Lane 3: human heart lysates Lane 4: mouse liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

•  

   
     Overlay histogram showing ZF4 cells stained with P33276 (green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33276,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

•  

   
     P33276 staining Zebrafish ak2 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

•  

   
     P33276 staining Zebrafish ak2 in zebra fish body tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The zebrafish *ak2* antibody is a tool used to study the function and expression of adenylate kinase 2 (AK2) in zebrafish (*Danio rerio*), a model organism widely employed in developmental biology, genetics, and disease research. AK2 is a mitochondrial enzyme critical for cellular energy homeostasis, catalyzing the reversible transfer of phosphate groups between ATP and AMP to maintain nucleotide balance. It plays roles in apoptosis, differentiation, and immune cell development. In zebrafish, AK2 is implicated in early embryonic development, particularly in tissues with high energy demands, such as the brain, heart, and musculature.

The antibody is typically generated against conserved regions of zebrafish AK2. allowing detection of endogenous protein expression via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Its development stems from interest in modeling human AK2-related disorders, such as reticular dysgenesis (a severe immunodeficiency), in zebrafish. Studies using this antibody have explored AK2's role in mitochondrial dysfunction, metabolic regulation, and hematopoietic defects. Validation often includes knockout models or knockdown experiments (e.g., morpholinos) to confirm specificity. Researchers leverage this tool to dissect molecular mechanisms underlying energy metabolism disorders and developmental anomalies, bridging insights from zebrafish to human pathophysiology.