| WB | 1/2000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | SCL-interrupting locus protein homolog, stil, sill |
| Entrez GeneID | 192317 |
| WB Predicted band size | 138.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This Zebrafish stil antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 866-900 amino acids from the C-terminal region of Zebrafish stil. |
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以下是关于斑马鱼Stil抗体的示例参考文献(注：以下文献信息为虚构示例，实际文献需通过学术数据库检索获取)：
1. **"Functional analysis of Stil in zebrafish neural tube development"**
*作者：Zhang Y, Li H, Wang J*
摘要：本研究利用斑马鱼模型，通过Stil特异性抗体进行免疫组化分析，揭示了Stil蛋白在胚胎神经管发育中的动态表达模式。结果显示，Stil缺失导致神经祖细胞增殖异常，提示其在Shh信号通路调控中起关键作用。
2. **"Generation and validation of a polyclonal antibody against zebrafish Stil for protein localization studies"**
*作者：Chen X, Tanaka M, Liu R*
摘要：文章报道了一种新型兔源多克隆抗体的开发，该抗体针对斑马鱼Stil蛋白C端结构域。通过Western blot和免疫荧光验证其特异性，并应用于胚胎发育过程中Stil的亚细胞定位研究。
3. **"Stil-mediated centrosome amplification in zebrafish cancer models"**
*作者：Gupta S, Patel T, Torres E*
摘要：研究利用斑马鱼肿瘤模型，结合Stil抗体进行免疫沉淀和质谱分析，发现Stil过表达导致中心体扩增，促进基因组不稳定性，为Stil在癌症发生中的作用提供了机制解释。
4. **"CRISPR/Cas9 knockout and antibody-based tracking of Stil in zebrafish gastrulation"**
*作者：Kim J, Park S, Lee W*
摘要：通过CRISPR/Cas9技术构建Stil突变体斑马鱼，并利用特异性抗体追踪蛋白表达，发现Stil缺失影响原肠胚形成阶段的细胞迁移和命运决定。
**建议**：实际文献可通过PubMed、Google Scholar等平台搜索关键词“zebrafish Stil antibody”“Danio rerio Stil protein”获取。关注《Development》《PLOS Genetics》等期刊的相关研究。
The zebrafish Stil (SCL/TAL1 interrupting locus) protein is a centrosomal component critical for regulating cell cycle progression, ciliogenesis, and Hedgehog (Hh) signaling during embryonic development. Stil is evolutionarily conserved, with homologs in humans (STIL) and model organisms, and plays a key role in centriole duplication by interacting with components like Sas-6 and CPAP. In zebrafish, Stil is essential for proper neurogenesis, left-right patterning, and organogenesis, particularly in tissues dependent on Hh signaling, such as the brain and somites. Mutations in Stil are linked to developmental disorders like microcephaly and primary microcephaly (MCPH) in humans, making zebrafish a valuable model to study these conditions.
Antibodies against zebrafish Stil are primarily used to investigate its spatial-temporal expression, subcellular localization (e.g., centrosomes or cilia), and functional roles in developmental processes. These tools enable visualization of Stil dynamics during cell division, cilia assembly, or Hh pathway activation via techniques like immunofluorescence, Western blotting, or immunohistochemistry. Studies using Stil antibodies in zebrafish have elucidated its involvement in neural progenitor proliferation, cilia-related defects, and cancer models (e.g., medulloblastoma), where Stil overexpression is oncogenic. Such research bridges developmental biology and disease mechanisms, highlighting Stil's dual role in normal development and pathology.
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