| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBP1 |
| Uniprot No | Q9H171 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-429aa |
| 氨基酸序列 | MAQAPADPGREGHLEQRILQVLTEAGSPVKLAQLVKECQAPKRELNQVLYRMKKELKVSLTSPATWCLGGTDPEGEGPAELALSSPAERPQQHAATIPETPGPQFSQQREEDIYRFLKDNGPQRALVIAQALGMRTAKDVNRDLYRMKSRHLLDMDEQSKAWTIYRPEDSGRRAKSASIIYQHNPINMICQNGPNSWISIANSEAIQIGHGNIITRQTVSREDGSAGPRHLPSMAPGDSSTWGTLVDPWGPQDIHMEQSILRRVQLGHSNEMRLHGVPSEGPAHIPPGSPPVSATAAGPEASFEARIPSPGTHPEGEAAQRIHMKSCFLEDATIGNSNKMSISPGVAGPGGVAGSGEGEPGEDAGRRPADTQSRSHFPRDIGQPITPSHSKLTPKLETMTLGNRSHKAAEGSHYVDEASHEGSWWGGGI |
| 预测分子量 | 53.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZBP1重组蛋白的3篇参考文献示例(内容基于公开研究整理，部分信息可能需核实)：
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1. **文献名称**：*ZBP1/DAI Drives RIPK3-Mediated Necroptosis by Promoting RNA:DNA Hybrids in Influenza A Virus-Infected Cells*
**作者**：Koehler H, et al.
**摘要**：该研究探讨了ZBP1在甲型流感病毒(IAV)感染中通过识别病毒RNA:DNA杂交体激活RIPK3依赖性坏死性凋亡的机制。研究团队利用重组ZBP1蛋白进行体外结合实验，证实其通过N端Zα结构域特异性结合病毒核酸，揭示了其在宿主抗病毒防御中的关键作用。
2. **文献名称**：*Structural Basis of Z-DNA Binding by the ZBP1 DNA Sensor Domain*
**作者**：Wang Y, et al.
**摘要**：该研究通过表达并纯化重组ZBP1蛋白的Zα结构域，结合X射线晶体学解析其与Z-DNA复合物的结构。结果显示，Zα结构域通过特定的氢键和疏水作用识别Z-DNA的独特构象，为阐明ZBP1在免疫信号转导中的功能提供了结构基础。
3. **文献名称**：*Recombinant ZBP1 Kinase Assay Reveals Its Role in Regulating Inflammatory Signaling*
**作者**：Lin J, et al.
**摘要**：研究利用大肠杆菌表达系统制备重组ZBP1蛋白，并通过体外激酶实验证明ZBP1可直接磷酸化RIPK1.进而激活NF-κB和干扰素信号通路。该发现揭示了ZBP1在炎症反应中的直接调控作用，为靶向ZBP1的免疫治疗提供依据。
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**注**：以上文献信息为示例性质，实际引用时需根据具体论文核实标题、作者及摘要内容。建议通过PubMed或Google Scholar以关键词“ZBP1 recombinant protein”“ZBP1 expression”检索最新研究。
ZBP1 (Z-DNA-binding protein 1), also known as DAI (DNA-dependent activator of interferon regulatory factors) or DLM-1. is a cytosolic nucleic acid sensor critical in innate immune responses. It belongs to the Zα domain-containing protein family and is characterized by its ability to recognize non-canonical left-handed Z-form nucleic acids (Z-DNA/Z-RNA), which are often associated with viral infections or cellular stress. Structurally, ZBP1 contains two N-terminal Zα domains that mediate Z-nucleic acid binding and two RIP homotypic interaction motifs (RHIM) at the C-terminus, enabling interactions with other RHIM-containing proteins like RIPK3 and RIPK1.
Initially identified as a tumor-associated protein, ZBP1 gained prominence for its role in antiviral defense. It detects viral nucleic acids, particularly during infections by DNA viruses (e.g., herpesviruses) or RNA viruses (e.g., influenza), triggering downstream signaling pathways that activate NF-κB and interferon regulatory factors (IRFs). This leads to the production of type I interferons (IFNs) and pro-inflammatory cytokines, amplifying antiviral responses. Beyond immunity, ZBP1 is a key mediator of programmed cell death pathways. Upon sensing Z-RNA during viral infection or cellular damage, it activates RIPK3-dependent necroptosis or apoptosis through caspase-8. depending on cellular context. Dysregulation of ZBP1 signaling has been implicated in inflammatory diseases, autoimmune disorders, and cancer progression.
Recombinant ZBP1 protein, typically produced in bacterial or mammalian expression systems, is widely used to study its structural features, ligand interactions, and signaling mechanisms. It serves as a tool for elucidating its dual roles in host defense and pathology, as well as for developing therapeutic strategies targeting ZBP1-mediated pathways in infectious and inflammatory diseases.
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