| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA4A |
| Uniprot No | Q9H3S1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-683aa |
| 氨基酸序列 | GGGQGPMPRVRYYAGDERRALSFFHQKGLQDFDTLLLSGDGNTLYVGARE AILALDIQDPGVPRLKNMIPWPASDRKKSECAFKKKSNETQCFNFIRVLV SYNVTHLYTCGTFAFSPACTFIELQDSYLLPISEDKVMEGKGQSPFDPAH KHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDNFLRWLHHDAS FVAAIPSTQVVYFFFEETASEFDFFERLHTSRVARVCKNDVGGEKLLQKK WTTFLKAQLLCTQPGQLPFNVIRHAVLLPADSPTAPHIYAVFTSQWQVGG TRSSAVCAFSLLDIERVFKGKYKELNKETSRWTTYRGPETNPRPGSCSVG PSSDKALTFMKDHFLMDEQVVGTPLLVKSGVEYTRLAVETAQGLDGHSHL VMYLGTTTGSLHKAVVSGDSSAHLVEEIQLFPDPEPVRNLQLAPTQGAVF VGFSGGVWRVPRANCSVYESCVDCVLARDPHCAWDPESRTCCLLSAPNLN SWKQDMERGNPEWACASGPMSRSLRPQSRPQIIKEVLAVPNSILELPCPH LSALASYYWSHGPAAVPEASSTVYNGSLLLIVQDGVGGLYQCWATENGFS YPVISYWVDSQDQTLALDPELAGIPREHVKVPLTRVSGGAALAAQQSYWP H |
| 预测分子量 | 72 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMA4A重组蛋白的3篇代表性文献，涵盖其在不同领域的生物学功能研究：
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1. **文献名称**：Semaphorin 4A interacts with TIM-2 and functions as a negative regulator of T cell activation
**作者**：Kumanogoh A. et al.
**摘要**：该研究利用重组SEMA4A蛋白，揭示了其通过结合T细胞表面的TIM-2受体抑制T细胞活化和增殖的机制，为SEMA4A在免疫调节中的作用提供了实验依据。
2. **文献名称**：Semaphorin 4A inhibits angiogenic proliferation and migration in vitro via VEGF receptor 2 downregulation
**作者**：Toyofuku T. et al.
**摘要**：通过体外实验，重组SEMA4A蛋白被证明可下调血管内皮细胞中的VEGFR2信号通路，显著抑制血管生成，提示其在抗肿瘤治疗中的潜在应用价值。
3. **文献名称**：Structural basis of semaphorin-plexin recognition and viral mimicry from Sema7A and A39R complexes with PlexinC1
**作者**：Love C.A. et al.
**摘要**：该研究解析了SEMA4A重组蛋白的晶体结构，阐明了其与Plexin受体结合的分子机制，为设计靶向SEMA4A信号通路的药物提供了结构基础。
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以上文献涉及SEMA4A在免疫调节、血管生成及结构生物学中的关键研究，展示了重组蛋白在功能探索中的广泛应用。如需具体发表年份或期刊，建议通过PubMed或Google Scholar进一步检索。
**Background of SEMA4A Recombinant Protein**
Semaphorin 4A (SEMA4A), a member of the semaphorin family, is a transmembrane or secreted glycoprotein that regulates cellular communication through interactions with receptors such as Plexin-B1. Plexin-D1. and Tim-2. It plays critical roles in immune regulation, neural development, angiogenesis, and tumorigenesis. SEMA4A is involved in modulating T-cell activation, dendritic cell function, and Th1/Th2 immune balance, making it a key player in autoimmune diseases, allergies, and cancer immunotherapy. In the nervous system, it guides axon pathfinding and neuronal connectivity, while in vascular biology, it influences endothelial cell migration and blood vessel formation.
Recombinant SEMA4A protein is engineered to mimic the native protein’s functional domains, typically produced in mammalian or insect cell systems to ensure proper folding and post-translational modifications. This purified protein retains bioactivity for *in vitro* and *in vivo* studies, enabling researchers to explore its signaling pathways (e.g., PI3K/AKT, MAPK) and therapeutic potential.
In disease contexts, SEMA4A exhibits dual roles. It acts as a tumor suppressor in some cancers by inhibiting metastasis but may promote angiogenesis or immune evasion in others. Its recombinant form is used to study mechanisms in autoimmune disorders like multiple sclerosis and rheumatoid arthritis. Additionally, SEMA4A is investigated as a biomarker for disease progression or a therapeutic target, with emerging interest in blocking its interaction with immune checkpoints (e.g., PD-1) to enhance anti-tumor responses.
Overall, SEMA4A recombinant protein serves as a vital tool for dissecting its multifunctional biology and developing targeted therapies across immunology, oncology, and neurology.
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