Recombinant Human XPNPEP3 protein

中文名： 氨基肽酶P3(XPNPEP3)重组蛋白
别名： XPNPEP3；Xaa-Pro aminopeptidase 3

货号: PA1000-4964

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	XPNPEP3
Uniprot No	Q9NQH7
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-507aa
氨基酸序列	MGSSHHHHHHSSGLVPRGSHMPWLLSAPKLVPAVANVRGLSGCMLCSQRR YSLQPVPERRIPNRYLGQPSPFTHPHLLRPGEVTPGLSQVEYALRRHKLM SLIQKEAQGQSGTDQTVVVLSNPTYYMSNDIPYTFHQDNNFLYLCGFQEP DSILVLQSLPGKQLPSHKAILFVPRRDPSRELWDGPRSGTDGAIALTGVD EAYTLEEFQHLLPKMKAETNMVWYDWMRPSHAQLHSDYMQPLTEAKAKSK NKVRGVQQLIQRLRLIKSPAEIERMQIAGKLTSQAFIETMFTSKAPVEEA FLYAKFEFECRARGADILAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLL DGGCESSCYVSDITRTWPVNGRFTAPQAELYEAVLEIQRDCLALCFPGTS LENIYSMMLTLIGQKLKDLGIMKNIKENNAFKAARKYCPHHVGHYLGMDV HDTPDMPRSLPLQPGMVITIEPGIYIPEDDKDAPEKFRGLGVRIEDDVVV TQDSPFILSADCPKEMNDIEQICSQASLEHHHHHH
预测分子量	60 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于XPNPEP3重组蛋白的3篇参考文献(注：文献为虚构示例，仅用于演示格式)：

1. **文献名称**：*Recombinant XPNPEP3 Expression and Enzymatic Characterization in Mitochondrial Dysfunction*

**作者**：Smith A, et al.

**摘要**：本研究成功在大肠杆菌中表达并纯化了重组XPNPEP3蛋白，证实其具有脯氨酸特异性氨基肽酶活性，并发现其在调节线粒体肽代谢中的作用缺失与肾小管病变相关。

2. **文献名称**：*Structural Insights into XPNPEP3 Mutations Associated with Nephronophthisis*

**作者**：Chen L, et al.

**摘要**：通过X射线晶体学解析了重组人源XPNPEP3的三维结构，揭示了突变位点如何破坏蛋白稳定性及底物结合能力，为遗传性肾病机制提供分子基础。

3. **文献名称**：*Functional Analysis of XPNPEP3 in Ciliopathy Models Using Recombinant Protein Complementation*

**作者**：Wang Y, et al.

**摘要**：利用重组XPNPEP3蛋白恢复纤毛缺陷细胞模型的功能，证明其通过水解特定肽链调控纤毛信号通路，为治疗纤毛病提供潜在策略。

(注：实际文献需通过PubMed、Google Scholar等平台检索。)

背景信息

**Background on XPNPEP3 Recombinant Protein**

XPNPEP3. or X-prolyl aminopeptidase 3. is a mitochondrial protein encoded by the *XPNPEP3* gene in humans. It belongs to the M24 peptidase family and is implicated in the cleavage of N-terminal Xaa-Pro bonds in peptides, a reaction critical for regulating bioactive molecules like collagen fragments, inflammatory mediators, and hormones. Unlike its cytosolic counterpart XPNPEP1. XPNPEP3 is anchored to the mitochondrial inner membrane, where it is hypothesized to participate in mitochondrial peptide processing, energy metabolism, and homeostasis.

Recombinant XPNPEP3 protein is produced in vitro using expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its production often involves tagging for purification (e.g., His-tag) and optimizing conditions to retain enzymatic activity, given its dependence on metal cofactors like manganese. Research has linked *XPNPEP3* mutations to mitochondrial disorders, such as hepatocerebral mitochondrial DNA depletion syndrome, characterized by energy deficits and multi-organ dysfunction. This association underscores its role in mitochondrial tRNA processing and respiratory chain integrity.

Beyond disease mechanisms, recombinant XPNPEP3 is used to explore substrate specificity, inhibitor screening (e.g., for fibrosis or cancer therapies), and interactions with mitochondrial pathways. Its study also contributes to understanding post-translational modifications and peptide-driven signaling in cellular stress responses. Despite progress, its full substrate repertoire and regulatory networks remain under investigation, highlighting its potential as a therapeutic target or biomarker in metabolic and degenerative diseases.