| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLK4 |
| Uniprot No | Q9Y5K2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-254aa |
| 氨基酸序列 | SCSQIINGEDCSPHSQPWQAALVMENELFCSGVLVHPQWVLSAAHCFQNS YTIGLGLHSL EADQEPGSQMVEASLSVRHPEYNRPLLANDLMLIKLDESVSESDTIRSIS IASQCPTAGN SCLVSGWGLLANGRMPTVLQCVNVSVVSEEVCSKLYDPLYHPSMFCAGGG QDQKDSCNGD SGGPLICNGYLQGLVSFGKAPCGQVGVPGVYTNLCKFTEWIEKTVQASVD HHHHHH |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLK4重组蛋白的3篇示例参考文献(内容为模拟概括，仅供参考)：
1. **《Recombinant human kallikrein 4 expression and enzymatic characterization》**
*作者：Debela M, et al.*
摘要：研究通过昆虫细胞表达系统成功制备重组人KLK4蛋白，并对其酶活性进行表征，发现其在酸性pH下对牙釉质基质蛋白具有特异性水解功能，提示其在牙釉质成熟中的关键作用。
2. **《Proteolytic activation of protease-activated receptor 1 by kallikrein-related peptidase 4》**
*作者：Ramsay AJ, et al.*
摘要：报道了重组KLK4蛋白能够通过切割激活蛋白酶活化受体1(PAR1)，促进前列腺癌细胞迁移和侵袭，揭示了KLK4在肿瘤微环境中的潜在信号调控机制。
3. **《Structural insights into KLK4 activation and substrate specificity》**
*作者：Elliott MB, et al.*
摘要：利用重组KLK4的晶体结构解析，阐明其酶原激活过程及底物结合口袋的构象变化，为设计特异性抑制剂治疗KLK4相关疾病(如牙发育异常、癌症转移)提供结构基础。
*注：以上文献信息为示例，实际引用时建议通过PubMed或学术数据库核实具体文献。*
**Background of Recombinant KLK4 Protein**
Kallikrein-related peptidase 4 (KLK4), a member of the serine protease family, is encoded by the *KLK4* gene and plays critical roles in tissue remodeling, enamel formation, and cancer progression. Initially identified for its involvement in dental enamel maturation, KLK4 cleaves enamel matrix proteins during tooth development, ensuring proper mineralization. Beyond dentistry, KLK4 is implicated in prostate and ovarian cancers, where its overexpression correlates with tumor invasion, metastasis, and poor prognosis, likely due to its ability to degrade extracellular matrix components and activate signaling pathways.
Recombinant KLK4 protein is engineered via genetic engineering techniques, typically expressed in mammalian or bacterial systems to ensure proper post-translational modifications (e.g., glycosylation) and functional activity. Its production enables detailed biochemical studies, including substrate specificity, enzymatic kinetics, and interactions with inhibitors. Researchers utilize recombinant KLK4 to explore its pathophysiological roles, particularly in cancer biology and enamel disorders, as well as to develop diagnostic tools or therapeutic agents. For instance, KLK4 inhibitors are being investigated as potential anticancer drugs.
Structurally, KLK4 shares a conserved catalytic triad (His, Asp, Ser) with other kallikreins but exhibits unique substrate preferences. Its activity is regulated by endogenous inhibitors (e.g., serpins) and pH-dependent zymogen activation. Recent studies also highlight its potential as a biomarker for prostate cancer, aiding in early detection and monitoring.
In summary, recombinant KLK4 serves as a vital tool for unraveling the protease's dual roles in physiology and disease, bridging translational research from basic enzymology to therapeutic innovation.
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