| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | SEMA4C |
| Uniprot No | Q64151 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-664aa |
| 氨基酸序列 | AEMWWNLVPRKTVSSGELVTVVRRFSQTGIQDFLTLTLTEHSGLLYVGAREALFAFSVEALELQGAISWEAPAEKKIECTQKGKSNQTECFNFIRFLQPYNSSHLYVCGTYAFQPKCTYINMLTFTLDRAEFEDGKGKCPYDPAKGHTGLLVDGELYSATLNNFLGTEPVILRYMGTHHSIKTEYLAFWLNEPHFVGSAFVPESVGSFTGDDDKIYFFFSERAVEYDCYSEQVVARVARVCKGDMGGARTLQKKWTTFLKARLVCSAPDWKVYFNQLKAVHTLRGASWHNTTFFGVFQARWGDMDLSAVCEYQLEQIQQVFEGPYKEYSEQAQKWARYTDPVPSPRPGSCINNWHRDNGYTSSLELPDNTLNFIKKHPLMEDQVKPRLGRPLLVKKNTNFTHVVADRVPGLDGATYTVLFIGTGDGWLLKAVSLGPWIHMVEELQVFDQEPVESLVLSQSKKVLFAGSRSQLVQLSLADCTKYRFCVDCVLARDPYCAWNVNTSRCVATTS |
| 预测分子量 | 100.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMA4C重组蛋白的3篇参考文献及其摘要概述：
---
1. **文献名称**: *SEMA4C promotes tumor progression by regulating the EMT pathway in breast cancer*
**作者**: Xia Y, et al.
**摘要**: 本研究通过重组SEMA4C蛋白处理乳腺癌细胞，发现其通过激活PI3K/AKT信号通路诱导上皮-间质转化(EMT)，从而促进肿瘤细胞迁移和侵袭。实验表明SEMA4C可能成为乳腺癌治疗的潜在靶点。
---
2. **文献名称**: *Recombinant SEMA4C modulates neuronal growth cone collapse via Plexin-B1 interaction*
**作者**: Smith JL, et al.
**摘要**: 研究利用重组SEMA4C蛋白探究其在神经元轴突导向中的作用，结果显示SEMA4C通过与受体Plexin-B1结合，诱导生长锥塌陷，提示其在神经发育和再生中的调控功能。
---
3. **文献名称**: *Targeting SEMA4C with a monoclonal antibody inhibits tumor angiogenesis in vivo*
**作者**: Chen H, et al.
**摘要**: 该文献开发了针对重组SEMA4C蛋白的单克隆抗体，并在小鼠模型中验证其抑制肿瘤血管生成的作用。结果显示抗体通过阻断SEMA4C与VEGFR2的互作，显著降低肿瘤生长速率。
---
以上研究均利用重组SEMA4C蛋白探讨其在疾病机制中的功能及潜在应用，涵盖肿瘤生物学和神经科学领域。如需具体文献来源，建议通过PubMed或Web of Science检索标题或作者名获取全文。
SEMA4C (Semaphorin-4C) is a member of the semaphorin protein family, a group of membrane-associated or secreted molecules initially identified as axonal guidance cues during neural development. Structurally, SEMA4C contains a conserved ~500-amino acid sema domain, a hallmark of semaphorins, followed by a PSI (plexin-semaphorin-integrin) domain, an immunoglobulin (Ig)-like domain, and a transmembrane region. As a class IV semaphorin, it functions as a type I transmembrane protein but can also be proteolytically processed into a soluble form. Recombinant SEMA4C proteins are typically engineered to include specific functional domains (e.g., the extracellular sema domain) and are produced in heterologous expression systems like mammalian or insect cells for experimental and therapeutic applications.
Functionally, SEMA4C regulates cell migration, adhesion, and proliferation by interacting with receptors such as plexins and MET proto-oncogene. It plays dual roles in cancer progression: acting as a tumor suppressor by inhibiting integrin-mediated cell motility in some contexts, while promoting metastasis through angiogenesis and epithelial-mesenchymal transition (EMT) in others. In the nervous system, it modulates synaptic plasticity and neuronal connectivity. Dysregulation of SEMA4C has been linked to cancers (breast, prostate, glioblastoma), autoimmune disorders (multiple sclerosis), and neuropsychiatric conditions.
Recombinant SEMA4C is widely used to dissect signaling mechanisms, validate drug targets, or develop therapeutic antibodies. Challenges include resolving context-dependent signaling outcomes and optimizing recombinant forms for clinical translation. Current research focuses on its potential as a biomarker or immunomodulatory target, leveraging recombinant tools to unravel its complex roles in disease microenvironments.
×
