| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WBP1 |
| Uniprot No | Q96G27 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 170-269aa |
| 氨基酸序列 | GTNVEGVSSHQSAPPHQEGEPGAGVTPASTPPSCRYRRLTGDSGIELCPC PASGEGEPVKEVRVSATLPDLEDYSPCALPPESVPQIFPMGLSSSEGDIP |
| 预测分子量 | 10 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WBP1重组蛋白的3篇参考文献示例(内容为模拟概括，仅供参考)：
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1. **文献名称**：*WBP1 interacts with E6AP and facilitates ubiquitination of the human papillomavirus oncoprotein E6*
**作者**：Chen C, et al.
**摘要**：研究通过重组WBP1蛋白体外实验，揭示了其与E6AP泛素连接酶的相互作用机制，证明WBP1在HPV病毒E6蛋白泛素化降解过程中起关键辅助作用。
2. **文献名称**：*Expression and functional analysis of recombinant WBP1 in yeast two-hybrid screening*
**作者**：Bedford MT, et al.
**摘要**：报道了在大肠杆菌中高效表达并纯化重组WBP1蛋白的方法，利用该蛋白进行酵母双杂交筛选，发现其WW结构域特异性结合富含脯氨酸的蛋白基序。
3. **文献名称**：*Structural characterization of the WW domain-binding protein 1 (WBP1) by NMR spectroscopy*
**作者**：Macias MJ, et al.
**摘要**：通过核磁共振技术解析了重组WBP1蛋白的WW结构域三维结构，阐明了其介导蛋白质相互作用的分子基础，为设计靶向抑制剂提供结构依据。
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**注**：以上文献信息为示例，实际引用需根据具体研究核实原文内容及作者信息。
WBP1 (WW Domain-Binding Protein 1), also known as PAWR or PRKCABP, is a multifunctional protein involved in diverse cellular processes, including apoptosis, transcriptional regulation, and protein-protein interactions. It was initially identified through its interaction with the WW domain of the Yes-associated protein (YAP), a key regulator of the Hippo signaling pathway. Structurally, WBP1 contains multiple functional domains, such as a proline-rich region, a WW domain-binding motif, and a C-terminal PDZ-binding domain, enabling it to act as an adaptor molecule in signaling networks. Its role in apoptosis is particularly notable, as it can promote cell death by interacting with proteins like PKC and Fas, while also modulating pathways like the PI3K/AKT and MAPK cascades.
Recombinant WBP1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, biologically active forms for research. This allows precise study of its molecular interactions and mechanisms. Studies highlight WBP1's dual role in cancer, where it can act as a tumor suppressor by inducing apoptosis in certain contexts but may also exhibit oncogenic properties depending on cellular conditions. Dysregulation of WBP1 has been linked to cancers, neurodegenerative diseases, and immune disorders, making it a potential therapeutic target. Recombinant WBP1 is widely used in biochemical assays, structural studies, and drug discovery to explore its regulatory functions and develop targeted therapies. Its ability to influence critical pathways underscores its importance in both basic research and translational medicine.
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