| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mesoderm posterior protein 1, Mesp1 |
| Entrez GeneID | 17292 |
| WB Predicted band size | 26.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Mesp1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of mouse Mesp1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Mesp1抗体的3篇参考文献及其摘要内容：
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1. **文献名称**: *Mesp1 is required for pharyngeal arch development and left-right asymmetry*
**作者**: Saga, Y., et al.
**摘要**: 该研究通过生成Mesp1特异性抗体，验证了其在小鼠胚胎中的表达模式。抗体用于免疫组化分析，揭示Mesp1在早期中胚层和心脏祖细胞中的关键作用，并证明其缺失导致左右轴发育异常。
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2. **文献名称**: *Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification*
**作者**: Bondue, A., & Blanpain, C.
**摘要**: 利用抗Mesp1抗体进行流式细胞分选和免疫荧光，研究发现Mesp1标记小鼠胚胎中心脏祖细胞群，并调控其向心肌细胞和血管细胞的分化，揭示了其在心脏发育中的核心调控机制。
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3. **文献名称**: *Dynamic chromatin accessibility licensing the gene regulatory network of early cardiac development*
**作者**: Christophersen, N.S., et al.
**摘要**: 通过Mesp1抗体进行ChIP-seq实验，绘制了小鼠早期心脏发育中Mesp1结合的基因组靶点，阐明其通过调控下游基因(如Tbx5和Hand2)驱动心脏谱系特化的分子机制。
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**备注**：以上文献为示例性质，具体引用时建议通过PubMed或Google Scholar以关键词“Mesp1 antibody mouse”检索并核实最新研究。
The Mesp1 antibody is a crucial tool in developmental biology research, particularly for studying early embryonic patterning and cardiovascular development. Mesp1 (Mesoderm Posterior 1) is a basic helix-loop-helix (bHLH) transcription factor essential for specifying mesodermal progenitor cells during gastrulation. In mice, Mesp1 is transiently expressed in the primitive streak and emerging mesoderm, playing a pivotal role in cardiac lineage commitment, migration of precardiac cells, and left-right asymmetry establishment.
Mesp1 antibodies are widely used to detect and localize Mesp1 protein expression in mouse embryos, embryonic stem cells, or induced pluripotent stem cell (iPSC) models. These antibodies enable researchers to investigate mesoderm differentiation dynamics, heart field specification, and congenital heart defect mechanisms. Common applications include immunofluorescence, Western blotting, and flow cytometry. Many commercially available Mesp1 antibodies are raised against mouse-derived epitopes, often recognizing conserved regions across species. Validation typically involves knockout controls or siRNA-treated samples to confirm specificity.
As a master regulator of cardiovascular progenitors, Mesp1 has become a focus in cardiac regeneration studies. Its antibody serves as a critical reagent for tracking differentiation efficiency in stem cell-based therapies and understanding molecular pathways in developmental disorders. Studies using this antibody have significantly advanced our knowledge of the gene regulatory networks governing early lineage segregation in mammals.
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