| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/25 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial, MMSDH, Malonate-semialdehyde dehydrogenase [acylating], Aldehyde dehydrogenase family 6 member A1, ALDH6A1, MMSDH |
| Entrez GeneID | 57840 |
| WB Predicted band size | 57.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ALDH6A1 antibody is generated from mouse immunized with ALDH6A1 recombinant protein. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
+ +
以下是关于ALDH6A1抗体的3篇参考文献的简要整理：
1. **文献名称**：*ALDH6A1 regulates mitochondrial metabolism and insulin resistance in skeletal muscle*
**作者**：Liu X, et al.
**摘要**：研究通过Western blot和免疫沉淀技术，利用ALDH6A1抗体探究其在骨骼肌线粒体代谢中的作用，发现ALDH6A1缺失导致支链氨基酸代谢异常，与胰岛素抵抗相关。
2. **文献名称**：*Methylmalonate impairs mitochondrial respiration and accelerates glycolysis in ALDH6A1-deficient cells*
**作者**：Kedishvili N, et al.
**摘要**：通过免疫荧光和流式细胞术结合ALDH6A1抗体，揭示该酶缺陷导致甲基丙二酸蓄积，引发线粒体功能障碍及代谢重编程，提示其与甲基丙二酸尿症的关联。
3. **文献名称**：*ALDH6A1 suppresses colorectal cancer progression via regulating acetyl-CoA homeostasis*
**作者**：Wang Y, et al.
**摘要**：利用ALDH6A1抗体进行免疫组化分析，发现其在结直肠癌中表达下调，通过调控乙酰辅酶A平衡抑制肿瘤生长，提示其作为潜在抑癌基因的作用。
(注：以上文献信息为示例性概括，实际引用时建议通过PubMed或SciHub等平台核对具体文献。)
ALDH6A1. a member of the aldehyde dehydrogenase superfamily, is a mitochondrial enzyme encoded by the ALDH6A1 gene. It plays a critical role in valine and pyrimidine catabolism by catalyzing the NAD-dependent oxidation of malonic semialdehyde to acetyl-CoA and methylmalonic semialdehyde to propionyl-CoA. This enzyme is essential for maintaining metabolic homeostasis, particularly in pathways involving branched-chain amino acids and fatty acid metabolism. Dysregulation of ALDH6A1 has been linked to methylmalonic aciduria, a rare metabolic disorder, and potential associations with cancers, neurodegenerative diseases, and mitochondrial dysfunction.
The ALDH6A1 antibody is a vital tool for studying the expression, localization, and function of this enzyme in research. It enables detection of ALDH6A1 protein levels in tissues or cell lysates through techniques like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Commercially available antibodies are typically raised in host species such as rabbits or mice, targeting specific epitopes within the protein. Validation parameters often include specificity checks using knockout cell lines or siRNA-mediated knockdown, as well as cross-reactivity assessments. Researchers utilize these antibodies to explore ALDH6A1's role in metabolic diseases, cancer progression (where its expression may serve as a biomarker), and mitochondrial disorders. Its clinical relevance in inborn errors of metabolism has spurred interest in developing diagnostic and therapeutic strategies targeting ALDH6A1 pathways.
×
