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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INSL4 |
| Uniprot No | Q14641 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-139aa |
| 氨基酸序列 | AELRGCGPRFGKHLLSYCPMPEKTFTTTPGGWLLESGRPKEMVSTSNNKD GQALGTTSEFIPNLSPELKKPLSEGQPSLKKIILSRKKRSGRHRFDPFCC EVICDDGTSVKLCT |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于INSL4重组蛋白的3篇代表性文献，涵盖其功能、制备及应用研究：
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1. **文献名称**: *"Production of bioactive recombinant human INSL4 in mammalian cells"*
**作者**: Li Y, et al.
**摘要**: 该研究报道了在HEK293哺乳动物细胞中高效表达并纯化具有生物活性的人源INSL4重组蛋白。通过体外实验验证其与受体LRP6的结合能力，并证实其促进滋养层细胞迁移的功能，为研究INSL4在胚胎发育中的作用提供了工具。
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2. **文献名称**: *"INSL4 promotes invasion and metastasis of colorectal cancer through integrin signaling"*
**作者**: Zhang H, et al.
**摘要**: 研究者利用重组INSL4蛋白处理结肠癌细胞，发现其通过激活整合素-β1/FAK通路增强肿瘤细胞侵袭能力，提示INSL4可能作为癌症治疗的潜在靶点。
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3. **文献名称**: *"Structural and functional characterization of the INSL4-RXFP2 signaling axis"*
**作者**: Smith TP, et al.
**摘要**: 通过重组INSL4蛋白与受体RXFP2的共结晶分析，揭示了二者相互作用的分子机制，并发现INSL4在睾丸下降过程中的关键调控作用，为生殖系统疾病研究提供结构基础。
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注：以上文献信息为示例性内容，实际研究需通过学术数据库(如PubMed、Web of Science)检索确认。若需具体文献，建议使用关键词 **"INSL4 recombinant protein"** 或 **"INSL4 expression"** 进一步筛选。
**Background of INSL4 Recombinant Protein**
INSL4 (Insulin-like peptide 4), also known as early placenta insulin-like peptide (EPIL) or relaxin/insulin-like factor 2 (RLF2), is a member of the insulin/relaxin hormone family. It is encoded by the *INSL4* gene, located on human chromosome 9. and shares structural homology with insulin, relaxin, and other insulin-like peptides. INSL4 is primarily expressed in placental trophoblasts during early pregnancy, suggesting a role in placental development, though its exact biological functions remain less characterized compared to other family members like INSL3 or relaxin.
Structurally, INSL4 is synthesized as a preprohormone, which is proteolytically processed to form a mature peptide consisting of two chains (A and B) linked by disulfide bonds. This conformation is critical for receptor binding and signaling. While its receptor, RXFP2 (relaxin family peptide receptor 2), is shared with INSL3. studies indicate INSL4 may have unique signaling pathways or interactions, potentially influencing cell proliferation, apoptosis, and tissue remodeling.
Recombinant INSL4 protein is produced using biotechnological methods, often via bacterial or mammalian expression systems, to ensure proper folding and post-translational modifications. This engineered protein enables researchers to study INSL4’s physiological roles, particularly in reproduction, embryonic development, and cancer biology. For instance, aberrant INSL4 expression has been linked to gestational disorders, such as preeclampsia, and certain cancers (e.g., ovarian, breast), where it may act as a biomarker or therapeutic target.
Despite progress, INSL4 research remains in its early stages, with ongoing efforts to clarify its mechanisms and therapeutic potential. Recombinant INSL4 serves as a vital tool for unraveling its contributions to human health and disease.
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