关键信息
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基因名
SLC7A9
- 应用
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别名
SLC7A9；BAT1；b(0.+)-type amino acid transporter 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P82251
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表达区间
1-487aa
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氨基酸序列
MGDTGLRKRREDEKSIQSQEPKTTSLQKELGLISGISIIVGTIIGSGIFV SPKSVLSNTEAVGPCLIIWAACGVLATLGALCFAELGTMITKSGGEYPYL MEAYGPIPAYLFSWASLIVIKPTSFAIICLSFSEYVCAPFYVGCKPPQIV VKCLAAAAILFISTVNSLSVRLGSYVQNIFTAAKLVIVAIIIISGLVLLA QGNTKNFDNSFEGAQLSVGAISLAFYNGLWAYDGWNQLNYITEELRNPYR NLPLAIIIGIPLVTACYILMNVSYFTVMTATELLQSQAVAVTFGDRVLYP ASWIVPLFVAFSTIGAANGTCFTAGRLIYVAGREGHMLKVLSYISVRRLT PAPAIIFYGIIATIYIIPGDINSLVNYFSFAAWLFYGLTILGLIVMRFTR KELERPIKVPVVIPVLMTLISVFLVLAPIISKPTWEYLYCVLFILSGLLF YFLFVHYKFGWAQKISKPITMHLQMLMEVVPPEEDPE
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SLC7A9, a member of the solute carrier family, encodes a sodium-dependent cystine/glutamate transporter that plays a critical role in the uptake of cysteine and glutamate in various tissues, particularly in the kidneys and the central nervous system. This transporter is vital for maintaining cellular redox homeostasis and neurotransmitter balance, influencing numerous physiological processes. Dysregulation of SLC7A9 has been associated with various pathological conditions, including cystinuria—a genetic disorder leading to kidney stone formation—neurodegenerative diseases, and other metabolic disorders. To further understand its function and therapeutic potential, researchers have been focusing on the expression and characterization of recombinant SLC7A9 protein. This involves using various expression systems, such as bacterial or mammalian cells, to produce the protein in sufficient quantities for detailed functional studies, binding assays, and structural analysis. By elucidating the mechanistic insights into SLC7A9's transport activity and interactions with other ligands or proteins, these studies aim to pave the way for targeted interventions in related diseases and enhance our understanding of amino acid transport biology. The recombinant SLC7A9 protein serves as a crucial tool for both basic and applied biomedical research, facilitating the development of novel therapeutic approaches that could mitigate the impact of diseases linked to its dysfunction.
