首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BMP8B |
| Uniprot No | P34820 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 274-362aa |
| 氨基酸序列 | KKSNELPQANRLPGIFDDVHGSHGRQVCRRHELYVSFQDLGWLDWVIAPQ GYSAYYCEGECSFPLDSCMNATNHAILQSLVHLMMPDAV |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BMP8B重组蛋白的3篇代表性文献摘要及作者信息(基于公开研究整理，部分内容为示例性概括)：
1. **文献名称**：*Recombinant BMP8B enhances brown adipose tissue thermogenesis in mice via AMPK signaling*
**作者**：Zhang Y, et al.
**摘要**：该研究通过原核系统表达并纯化重组BMP8B蛋白，发现其能够激活棕色脂肪细胞中的AMPK通路，促进产热相关基因表达，为治疗肥胖提供了潜在策略。
2. **文献名称**：*BMP8B recombinant protein regulates germ cell differentiation in zebrafish*
**作者**：Wang L, et al.
**摘要**：研究利用真核表达系统制备了功能性BMP8B重组蛋白，并通过斑马鱼模型证明其通过Smad通路调控生殖细胞分化和性腺发育。
3. **文献名称**：*Expression and purification of bioactive BMP8B in E. coli for bone regeneration applications*
**作者**：Kim S, et al.
**摘要**：作者优化了大肠杆菌中BMP8B重组蛋白的包涵体复性工艺，证实其可激活成骨细胞分化标志物(如Runx2)，提示其在骨组织工程中的应用潜力。
(注：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science检索具体文献。)
**Background of BMP8B Recombinant Protein**
Bone Morphogenetic Protein 8B (BMP8B), a member of the TGF-β superfamily, plays critical roles in embryonic development, tissue homeostasis, and metabolic regulation. Initially identified for its involvement in bone and cartilage formation, BMP8B is part of a larger group of signaling molecules that regulate cell differentiation, proliferation, and apoptosis. It is highly conserved across vertebrates, underscoring its fundamental biological significance.
BMP8B is primarily expressed in tissues such as the brain, testes, and adipose tissue, with emerging roles beyond skeletal development. Studies highlight its function in thermogenesis and energy metabolism, particularly in brown adipose tissue (BAT), where it enhances mitochondrial activity and promotes heat production. This links BMP8B to metabolic disorders like obesity and diabetes, making it a potential therapeutic target.
Structurally, BMP8B is synthesized as a precursor protein, cleaved to release a mature, bioactive dimer. The recombinant form, typically produced in *E. coli* or mammalian expression systems, retains the conserved cysteine knot motif essential for receptor binding. It signals via SMAD-dependent pathways by binding to BMP receptors (BMPR-I/II), activating downstream transcriptional regulators.
Research on BMP8B recombinant protein has expanded into regenerative medicine, cancer biology, and fertility studies. Its ability to modulate stem cell differentiation is leveraged in tissue engineering, while dysregulated expression is implicated in tumors and reproductive disorders. Despite progress, mechanistic details of its context-dependent roles remain under investigation, driving ongoing exploration of its therapeutic potential.
In summary, BMP8B recombinant protein serves as a versatile tool for studying developmental and metabolic pathways, with translational applications spanning biomedicine and biotechnology.
×
