| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPQ |
| Uniprot No | Q9Y646 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-472aa |
| 氨基酸序列 | MKFLIFAFFGGVHLLSLCSGKAICKNGISKRTFEEIKEEIASCGDVAKAIINLAVYGKAQNRSYERLALLVDTVGPRLSGSKNLEKAIQIMYQNLQQDGLEKVHLEPVRIPHWERGEESAVMLEPRIHKIAILGLGSSIGTPPEGITAEVLVVTSFDELQRRASEARGKIVVYNQPYINYSRTVQYRTQGAVEAAKVGALASLIRSVASFSIYSPHTGIQEYQDGVPKIPTACITVEDAEMMSRMASHGIKIVIQLKMGAKTYPDTDSFNTVAEITGSKYPEQVVLVSGHLDSWDVGQGAMDDGGGAFISWEALSLIKDLGLRPKRTLRLVLWTAEEQGGVGAFQYYQLHKVNISNYSLVMESDAGTFLPTGLQFTGSEKARAIMEEVMSLLQPLNITQVLSHGEGTDINFWIQAGVPGASLLDDLYKYFFFHHSHGDTMTVMDPKQMNVAAAVWAVVSYVVADMEEMLPRS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CPQ(假设为某特定重组蛋白)的参考文献示例(注：部分文献为虚构示例，实际引用需通过学术数据库核实)：
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1. **文献名称**：*Expression and Purification of Recombinant CPQ Protein in E. coli*
**作者**：Zhang L, et al.
**摘要**：研究报道了通过大肠杆菌表达系统成功克隆并纯化CPQ重组蛋白，优化了诱导条件，并通过SDS-PAGE和Western blot验证蛋白活性，为后续功能研究提供基础。
2. **文献名称**：*Structural Analysis of CPQ Reveals Its Role in Cellular Signaling*
**作者**：Smith J, Patel R.
**摘要**：利用X射线晶体学解析了CPQ蛋白的三维结构，发现其与配体结合的活性位点，并揭示其在细胞信号转导通路中的潜在作用机制。
3. **文献名称**：*Functional Characterization of Recombinant CPQ in Metabolic Disorders*
**作者**：Kim S, et al.
**摘要**：通过体外实验证明重组CPQ蛋白可调节脂质代谢关键酶活性，提示其在代谢疾病治疗中的潜在应用价值。
4. **文献名称**：*High-Yield Production of CPQ Using Baculovirus Expression System*
**作者**：Garcia M, et al.
**摘要**：比较不同表达系统对CPQ产量的影响，发现昆虫杆状病毒系统可实现高效表达，且纯化蛋白具有更优的生物活性。
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**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台检索具体文献(如CPQ指代不明，建议确认蛋白全称或相关基因名)。
**Background of CPQ Recombinant Protein**
CPQ recombinant protein refers to a genetically engineered form of carboxypeptidase Q (CPQ), a proteolytic enzyme involved in selective cleavage of peptide bonds at the C-terminal end of proteins. Originally identified for its role in processing bioactive peptides and regulating protein function, CPQ has drawn interest in biopharmaceutical and industrial applications due to its substrate specificity and catalytic efficiency.
Recombinant CPQ is produced using microbial or mammalian expression systems. The gene encoding CPQ is cloned into a plasmid vector, transfected into host cells (e.g., *E. coli* or CHO cells), and expressed under controlled conditions. Post-expression, the protein is purified via chromatography to ensure high purity and activity. This method allows scalable production, overcoming limitations of extracting CPQ from natural sources.
Structurally, recombinant CPQ retains conserved domains critical for its enzymatic function, including a catalytic zinc-binding motif and substrate-binding pockets. Modifications, such as affinity tags or mutations, may be introduced to enhance stability, solubility, or compatibility with downstream applications.
Applications span multiple fields. In therapeutics, CPQ is explored for antibody-drug conjugate (ADC) processing or targeted protein degradation. Industrially, it serves as a biocatalyst for peptide synthesis or protein engineering. In research, it is a tool for studying post-translational modifications or generating precise protein fragments.
Challenges include optimizing expression yields, minimizing host cell contaminants, and maintaining activity during storage. Advances in protein engineering and fermentation technologies continue to address these hurdles, positioning CPQ recombinant protein as a versatile asset in biotechnology and medicine.
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