| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VASN |
| Uniprot No | Q6EMK4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-575aa |
| 氨基酸序列 | CPSGCQCSQPQTVFCTARQGTTVPRDVPPDTVGLYVFENGITMLDAGSFA GLPGLQLLDLSQNQIASLPSGVFQPLANLSNLDLTANRLHEITNETFRGL RRLERLYLGKNRIRHIQPGAFDTLDRLLELKLQDNELRALPPLRLPRLLL LDLSHNSLLALEPGILDTANVEALRLAGLGLQQLDEGLFSRLRNLHDLDV SDNQLERVPPVIRGLRGLTRLRLAGNTRIAQLRPEDLAGLAALQELDVSN LSLQALPGDLSGLFPRLRLLAAARNPFNCVCPLSWFGPWVRESHVTLASP EETRCHFPPKNAGRLLLELDYADFGCPATTTTATVPTTRPVVREPTALSS SLAPTWLSPTAPATEAPSPPSTAPPTVGPVPQPQDCPPSTCLNGGTCHLG TRHHLACLCPEGFTGLYCESQMGQGTRPSPTPVTPRPPRSLTLGIEPVSP TSLRVGLQRYLQGSSVQLRSLRLTYRNLSGPDKRLVTLRLPASLAEYTVT QLRPNATYSVCVMPLGPGRVPEGEEACGEAHTPPAVHSNHAPVTQAREGN LPVDHHHHHH |
| 预测分子量 | 61 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VASN(血管抑素，Vasorin)重组蛋白的3篇参考文献及其摘要概括：
1. **《Vasorin regulates arterial remodeling via TGF-β signaling》**
- 作者：Li Y, et al.
- 摘要：研究揭示VASN通过调控TGF-β信号通路参与动脉重塑过程，重组VASN蛋白在体外实验中抑制血管平滑肌细胞增殖和迁移，提示其潜在治疗动脉粥样硬化的应用价值。
2. **《Recombinant vasorin ameliorates liver fibrosis by antagonizing TGF-β1 in mice》**
- 作者：Zhang H, et al.
- 摘要：通过动物实验证明重组VASN蛋白可通过结合TGF-β1并阻断其下游信号通路，显著减轻小鼠肝纤维化程度，为肝病治疗提供新策略。
3. **《Structural and functional characterization of human vasorin extracellular domain》**
- 作者：Wang X, et al.
- 摘要：该研究解析了重组表达的人源VASN胞外域蛋白结构，发现其通过特定结构域与血管内皮生长因子(VEGF)相互作用，调控血管生成，为靶向药物设计奠定基础。
注：以上文献信息为示例性质，实际研究中请根据具体需求检索最新文献并核实准确性。
Vasorin (VASN), a transmembrane glycoprotein encoded by the VASN gene, plays multifaceted roles in cellular processes, including cell adhesion, signal transduction, and tissue remodeling. Initially identified as a regulator of vascular smooth muscle cell function, it modulates TGF-β signaling by binding to the ligand-receptor complex, thereby influencing fibrosis and angiogenesis. VASN is implicated in pathological conditions such as cancer metastasis, organ fibrosis, and cardiovascular diseases, making it a potential therapeutic target.
Recombinant VASN protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, bioactive forms for research. Its production typically involves cloning the VASN gene into expression vectors, optimizing codon usage, and employing affinity chromatography for purification. The recombinant protein retains functional domains critical for ligand interactions, enabling studies on its mechanistic roles in disease pathways.
In biomedical research, recombinant VASN aids in elucidating its role in epithelial-mesenchymal transition (EMT), tumor microenvironment modulation, and collagen deposition. It’s also utilized to develop neutralizing antibodies or inhibitors targeting VASN-driven pathways. Recent studies highlight its diagnostic potential as elevated VASN levels correlate with cancer progression and fibrotic severity. Despite its promise, challenges remain in understanding isoform-specific functions and optimizing recombinant protein stability for therapeutic applications. Ongoing research focuses on structure-activity relationships and preclinical validation to advance translational opportunities.
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