| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPP3 |
| Uniprot No | Q9NY33 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-737aa |
| 氨基酸序列 | ADTQYILPN DIGVSSLDCR EAFRLLSPTE RLYAYHLSRA AWYGGLAVLL QTSPEAPYIY ALLSRLFRAQ DPDQLRQHAL AEGLTEEEYQ AFLVYAAGVY SNMGNYKSFG DTKFVPNLPK EKLERVILGS EAAQQHPEEV RGLWQTCGEL MFSLEPRLRH LGLGKEGITT YFSGNCTMED AKLAQDFLDS QNLSAYNTRL FKEVDGEGKP YYEVRLASVL GSEPSLDSEV TSKLKSYEFR GSPFQVTRGD YAPILQKVVE QLEKAKAYAA NSHQGQMLAQ YIESFTQGSI EAHKRGSRFW IQDKGPIVES YIGFIESYRD PFGSRGEFEG FVAVVNKAMS AKFERLVASA EQLLKELPWP PTFEKDKFLT PDFTSLDVLT FAGSGIPAGI NIPNYDDLRQ TEGFKNVSLG NVLAVAYATQ REKLTFLEED DKDLYILWKG PSFDVQVGLH ELLGHGSGKL FVQDEKGAFN FDQETVINPE TGEQIQSWYR SGETWDSKFS TIASSYEECR AESVGLYLCL HPQVLEIFGF EGADAEDVIY VNWLNMVRAG LLALEFYTPE AFNWRQAHMQ ARFVILRVLL EAGEGLVTIT PTTGSDGRPD ARVRLDRSKI RSVGKPALER FLRRLQVLKS TGDVAGGRAL YEGYATVTDA PPECFLTLRD TVLLRKESRK LIVQPNTRLE GSDVQLLEYE ASAAGLIRSF SERFPEDGPE LEEILTQLAT ADARFWKGPS EAPSGQA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPP3重组蛋白的3篇参考文献示例(注：文献信息为模拟，仅供参考)：
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1. **文献名称**：*Recombinant Human DPP3 as a Potential Biomarker in Heart Failure*
**作者**：Souza, J.L. et al.
**摘要**：研究通过重组表达人源DPP3蛋白，分析了其在心力衰竭患者血浆中的酶活性水平，发现DPP3活性升高与心衰严重程度及不良预后显著相关，提示其作为新型生物标志物的潜力。
2. **文献名称**：*Crystal Structure of Recombinant DPP3 Reveals Substrate Binding Mechanism*
**作者**：Wagner, A. & Schmidt, F.
**摘要**：通过X射线晶体学解析重组DPP3蛋白的三维结构，揭示了其金属结合催化中心及底物识别区域，为设计特异性DPP3抑制剂提供了结构基础。
3. **文献名称**：*Role of Recombinant DPP3 in Oxidative Stress-Induced Apoptosis*
**作者**：Kumar, R. et al.
**摘要**：利用重组DPP3蛋白进行体外实验，发现其通过调节细胞内活性氧(ROS)水平及Caspase-3活性，参与氧化应激诱导的细胞凋亡通路调控。
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如需真实文献，建议在PubMed或Web of Science中检索关键词“recombinant DPP3 protein”或“DPP3 recombinant expression”。
**Background of Recombinant DPP3 Protein**
Dipeptidyl peptidase 3 (DPP3) is a zinc-dependent metalloprotease belonging to the M49 family, widely conserved across eukaryotes and prokaryotes. It catalyzes the hydrolysis of dipeptides from the N-terminus of oligopeptides, playing roles in peptide metabolism, cell signaling, and homeostasis. DPP3 is implicated in diverse physiological processes, including blood pressure regulation, pain modulation, and oxidative stress response, via its interaction with bioactive peptides such as angiotensin II, enkephalins, and glutathione.
Recombinant DPP3 protein is produced through genetic engineering, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its structure comprises two domains: a catalytic domain with a conserved zinc-binding motif (HEXXGH) and a substrate-binding domain. Dysregulation of DPP3 has been linked to pathologies like cancer, cardiovascular diseases, and neurodegenerative disorders, making it a target for therapeutic research.
In recent years, recombinant DPP3 has gained attention for its potential as a biomarker in critical care, as circulating DPP3 levels correlate with mortality in conditions like septic shock and heart failure. Additionally, its role in the Keap1-Nrf2 pathway—modulating cellular antioxidant responses—highlights its therapeutic relevance. Researchers utilize recombinant DPP3 to study enzyme kinetics, inhibitor screening, and molecular mechanisms underlying disease, driving drug development and diagnostic innovations.
Overall, recombinant DPP3 serves as a vital tool for unraveling its biological significance and translating findings into clinical applications.
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