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| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BMP5 |
| Uniprot No | P22003-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 324-454aa |
| 氨基酸序列 | QNRNKSSSHQ DSSRMSSVGD YNTSEQKQAC KKHELYVSFR DLGWQDWIIA PEGYAAFYCD GECSFPLNAH MNATNHAIVQ TLVHLMFPDH VPKPCCAPTK LNAISVLYFD DSSNVILKKY RNMVVRSCGC H |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BMP5重组蛋白的参考文献示例(内容为虚构示例，仅作格式参考)：
1. **"Recombinant Human BMP5 Promotes Osteoblast Differentiation In Vitro"**
*作者：King, J. et al.*
*摘要*：研究利用哺乳动物细胞系统表达重组人BMP5.并证实其通过激活SMAD通路增强间充质干细胞的成骨分化能力。
2. **"Structural Characterization of BMP5 Using X-ray Crystallography"**
*作者：Smith, R. & Lee, T.*
*摘要*：首次解析重组BMP5蛋白的晶体结构，揭示其与受体结合的关键位点，为靶向药物设计提供依据。
3. **"BMP5 Recombinant Protein Enhances Bone Regeneration in Rodent Models"**
*作者：Zhang, Y. et al.*
*摘要*：通过大鼠颅骨缺损模型验证重组BMP5的骨修复效果，显示其显著促进新骨形成，效果与BMP2相当。
4. **"Optimized Expression of BMP5 in E. coli for High-Yield Production"**
*作者：Chen, L. et al.*
*摘要*：开发大肠杆菌表达系统生产重组BMP5.优化纯化工艺并获得高活性蛋白，降低工业化生产成本。
(注：以上文献为示例，实际引用请通过PubMed或Web of Science检索真实研究。)
**Background of BMP5 Recombinant Protein**
Bone Morphogenetic Protein 5 (BMP5), a member of the transforming growth factor-beta (TGF-β) superfamily, plays critical roles in embryonic development, tissue homeostasis, and cellular processes such as proliferation, differentiation, and apoptosis. Discovered as part of the BMP family, BMP5 is involved in regulating skeletal formation, organogenesis, and cartilage development. It signals through serine/threonine kinase receptors, activating SMAD-dependent and non-SMAD pathways to modulate gene expression. Dysregulation of BMP5 has been linked to skeletal disorders, pulmonary diseases, and certain cancers, highlighting its biological and clinical significance.
Recombinant BMP5 protein is produced using genetic engineering techniques, typically via expression in mammalian cell systems (e.g., HEK293 or CHO cells) or *E. coli* to ensure proper folding and post-translational modifications. The purified protein consists of mature BMP5 dimers, often fused with tags (e.g., His-tag) for easier isolation. Rigorous quality control, including SDS-PAGE, Western blotting, and functional assays (e.g., ALP activity tests in mesenchymal cells), ensures bioactivity and purity.
As a research tool, recombinant BMP5 is widely used to study skeletal and developmental biology, disease mechanisms, and regenerative medicine. It also holds therapeutic potential for bone repair, cartilage regeneration, and targeted therapies for BMP pathway-related disorders. Its application in *in vitro* and *in vivo* models continues to advance understanding of TGF-β signaling and its translational prospects.
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