| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Long-chain specific acyl-CoA dehydrogenase, mitochondrial, LCAD, ACADL |
| Entrez GeneID | 33 |
| WB Predicted band size | 47.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ACADL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human ACADL. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ACADL(N端)抗体的3篇代表性文献示例(注：文献为假设性举例，实际引用需核实)：
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1. **文献名称**：*Characterization of a Novel N-terminal Specific Antibody for Long-chain Acyl-CoA Dehydrogenase (ACADL) in Cardiac Tissue*
**作者**：Smith J, et al.
**摘要**：本研究开发了一种针对ACADL蛋白N端表位的多克隆抗体，用于检测小鼠心肌组织中线粒体ACADL的表达。通过Western blot和免疫组化验证了抗体的特异性，并发现心力衰竭模型中ACADL表达显著下调。
2. **文献名称**：*Tissue-specific Distribution of ACADL in Mammalian Systems: Insights from N-terminal Antibody-based Analysis*
**作者**：Jones R, et al.
**摘要**：利用自主制备的ACADL N端单克隆抗体，分析了ACADL在肝脏、骨骼肌和脂肪组织中的表达差异。研究揭示了ACADL在长链脂肪酸氧化中的组织特异性调控机制。
3. **文献名称**：*ACADL Deficiency Screening Using N-terminal Targeted Antibodies in Human Fibroblasts*
**作者**：Lee H, et al.
**摘要**：通过基于ACADL N端抗体的免疫荧光技术，建立了一种快速诊断ACADL缺乏症的方法。该抗体可特异性识别患者细胞中ACADL的截短突变蛋白，为代谢性疾病诊断提供工具。
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**备注**：实际文献需通过数据库(如PubMed、Google Scholar)搜索关键词“ACADL antibody N-terminal”或联系抗体供应商获取引用。部分商业抗体的说明书可能附带相关参考文献。
ACADL (acyl-CoA dehydrogenase long chain), also known as LCAD, is a mitochondrial enzyme critical for fatty acid β-oxidation. It catalyzes the initial step of long-chain fatty acid breakdown, converting acyl-CoA derivatives into trans-enoyl-CoA while transferring electrons to the electron transfer flavoprotein (ETF). This process is essential for energy production, particularly in tissues with high metabolic demands like the heart, liver, and skeletal muscle. Dysregulation of ACADL has been linked to metabolic disorders, including mitochondrial dysfunction and lipid storage diseases.
The ACADL (N-term) antibody is a targeted immunoassay tool designed to recognize the N-terminal region of the ACADL protein. As an affinity-purified polyclonal or monoclonal antibody, it is commonly used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect ACADL expression levels, localization, and post-translational modifications. Researchers employ this antibody to investigate ACADL's role in metabolic homeostasis, its association with conditions like obesity, diabetes, and cardiomyopathies, or its potential as a biomarker in cancer studies. Validation typically includes knockout cell line controls to ensure specificity. The N-terminal region is often selected for antibody development due to its structural conservation across species and reduced likelihood of cross-reactivity with other acyl-CoA dehydrogenase family members. Its applications extend to both basic research and preclinical studies exploring therapeutic interventions targeting fatty acid metabolism.
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