Rabbit Polyclonal PLD6 Antibody

中文名： PLD6抗体
别名： Mitochondrial cardiolipin hydrolase, 31--, Choline phosphatase 6, Mitochondrial phospholipase, MitoPLD, Phosphatidylcholine-hydrolyzing phospholipase D6, Phospholipase D6, PLD 6, Protein zucchini homolog, PLD6

货号: IPDX32594

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/1000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	Mitochondrial cardiolipin hydrolase, 31--, Choline phosphatase 6, Mitochondrial phospholipase, MitoPLD, Phosphatidylcholine-hydrolyzing phospholipase D6, Phospholipase D6, PLD 6, Protein zucchini homolog, PLD6
Entrez GeneID	201164
WB Predicted band size	28.3kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This PLD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from of human PLD6.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

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Western blot analysis of lysates from human ovary and mouse testis tissue lysates (from left to right), using PLD6 Antibody (Center)(Cat. #P32594). P32594 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

参考文献

以下是关于PLD6抗体的3篇文献示例，包含文献名称、作者及摘要概括：

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1. **文献名称**：*PLD6 regulates mitochondrial dynamics and spermatogenesis*

**作者**：Zhang Y, et al.

**摘要**：该研究通过在小鼠模型中敲除PLD6.发现其缺失导致精子形成异常。利用PLD6抗体进行免疫荧光和Western blot实验，证实PLD6在线粒体膜上的定位，并参与线粒体融合-分裂平衡，影响生殖细胞发育。

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2. **文献名称**：*Mitochondrial phospholipase PLD6 promotes ovarian cancer cell metastasis via ROS-mediated AMPK signaling*

**作者**：Li X, Wang H, et al.

**摘要**：研究报道PLD6在卵巢癌细胞中高表达，通过PLD6抗体进行免疫组化分析发现其与患者预后不良相关。实验表明，PLD6通过ROS-AMPK通路增强癌细胞迁移，抗体检测证实其调控线粒体活性氧生成。

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3. **文献名称**：*PLD6 interacts with TEX19 to modulate piRNA biogenesis in germ cells*

**作者**：Huang N, et al.

**摘要**：本文利用PLD6抗体进行免疫共沉淀和质谱分析，发现PLD6与TEX19蛋白互作，共同调控piRNA生成。研究揭示了PLD6在生殖细胞中通过核酸酶活性影响转座子沉默的分子机制。

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**备注**：以上文献为示例，实际引用需根据具体研究补充完整信息(期刊、年份及DOI)。建议通过PubMed或Web of Science以“PLD6 antibody”为关键词检索最新文献。

背景信息

The phospholipase D6 (PLD6) antibody is a research tool designed to detect and study PLD6. a member of the phospholipase D (PLD) family. PLD6. also known as mitochondrial PLD or MitoPLD, is an enzyme that catalyzes the hydrolysis of phospholipids to generate phosphatidic acid, a lipid signaling molecule. Unlike other PLD isoforms, PLD6 is primarily localized to mitochondria, where it plays critical roles in mitochondrial dynamics, including membrane fusion, mitophagy, and regulation of mitochondrial RNA metabolism. It is also implicated in germ cell development, particularly spermatogenesis, through its involvement in piRNA (PIWI-interacting RNA) biogenesis, which safeguards genome integrity by silencing transposable elements.

PLD6 antibodies are widely used in molecular and cellular biology to investigate PLD6 expression, localization, and function in various tissues and disease models. They enable techniques like Western blotting, immunofluorescence, and immunohistochemistry. Dysregulation of PLD6 has been linked to infertility, neurodegenerative disorders, and cancer, making its study clinically relevant. For instance, PLD6 mutations or altered expression are associated with defects in mitochondrial function and germ cell maturation. Researchers also explore PLD6's role in metabolic syndromes and oxidative stress responses. As an emerging tool, PLD6 antibodies contribute to elucidating mitochondrial biology and potential therapeutic targets for related pathologies.