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| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSH1 |
| Uniprot No | P01243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-217aa |
| 氨基酸序列 | VQTVPLSRLFDHAMLQAHRAHQLAIDTYQEFEETYIPKDQKYSFLHDSQT SFCFSDSIPTPSNMEETQQKSNLELLRISLLLIESWLEPVRFLRSMFANN LVYDTSDSDDYHLLKDLEEGIQTLMGRLEDGSRRTGQILKQTYSKFDTNS HNHDALLKNYGLLYCFRKDMDKVETFLRMVQCRSVEGSCGFVDHHHHHH |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSH1重组蛋白的3篇参考文献的简要信息：
1. **文献名称**：*Expression and Purification of Recombinant Human Chorionic Somatomammotropin (CSH1) in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：该研究报道了通过大肠杆菌表达系统成功制备重组人CSH1蛋白，优化了诱导条件和纯化步骤，并验证了其与天然蛋白相似的免疫反应活性。
2. **文献名称**：*Functional Characterization of Recombinant CSH1 in Placental Development*
**作者**：Lee J, et al.
**摘要**：利用哺乳动物细胞表达系统获得重组CSH1.发现其通过激活JAK-STAT信号通路促进胎盘细胞增殖，并调节妊娠期胰岛素样生长因子分泌。
3. **文献名称**：*Structural Analysis and Receptor Binding of Recombinant CSH1*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析重组CSH1的三维结构，揭示其与生长激素受体的结合模式，为研究其生理功能及病理机制提供结构基础。
**备注**：上述文献为示例，实际研究中建议通过PubMed或Web of Science以关键词“CSH1 recombinant”或“chorionic somatomammotropin expression”检索最新文献。
CSH1 (chorionic somatomammotropin hormone 1), also known as human placental lactogen (hPL), is a peptide hormone produced predominantly by the syncytiotrophoblast cells of the placenta during pregnancy. It belongs to the growth hormone (GH) prolactin gene family and shares structural homology with both GH (85%) and prolactin. CSH1 is encoded by the CSH1 gene located on chromosome 17q23.3 within a cluster of five closely related genes, including GH and placental lactogen variants.
Functionally, CSH1 plays critical roles in maternal metabolic adaptation and fetal development. It induces insulin resistance in pregnant individuals, redirecting glucose toward the fetus while promoting lipolysis to provide alternative energy sources for the mother. Additionally, it stimulates mammary gland development and works synergistically with progesterone to maintain pregnancy. CSH1 levels rise progressively during gestation, correlating with placental mass, making it a clinical marker for placental function assessment.
Recombinant CSH1 protein is produced using biotechnological platforms such as bacterial (E. coli), yeast, or mammalian cell expression systems. The production typically involves gene cloning, protein expression, and purification steps like affinity chromatography. Recombinant versions retain the native hormone's biological activities while offering standardized quality and reduced batch variability compared to placenta-derived extracts.
Research applications include studying pregnancy-related metabolic disorders (e.g., gestational diabetes), fetal growth regulation mechanisms, and developing therapeutic strategies for placental insufficiency. Its mitogenic and metabolic effects are also explored in oncology and endocrinology research. As a recombinant protein, CSH1 provides a crucial tool for both basic research and potential clinical interventions in obstetrical complications.
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