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Rabbit Polyclonal EPC1 Antibody

  • 中文名: EPC1抗体
  • 别    名: Enhancer of polycomb homolog 1, EPC1
货号: IPDX32522
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesEnhancer of polycomb homolog 1, EPC1
Entrez GeneID80314
WB Predicted band size93.5kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis EPC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the Central region of human EPC1.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3-4条关于EPC1抗体的模拟参考文献(内容基于学术文献结构模拟,非真实存在):

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1. **文献名称**:*EPC1 Antibody-Based Detection of Polycomb Repressive Complexes in Cancer Cells*

**作者**:Smith A, et al.

**摘要**:研究开发了一种特异性识别EPC1蛋白的单克隆抗体,验证了其在染色质免疫沉淀(ChIP)和免疫荧光中的有效性,揭示了EPC1在多种癌症中与Polycomb复合物相互作用并调控基因沉默的机制。

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2. **文献名称**:*Functional Characterization of EPC1 in Epigenetic Regulation Using Knockout Models*

**作者**:Wang L, et al.

**摘要**:通过CRISPR/Cas9构建EPC1敲除细胞系,结合特异性抗体验证蛋白缺失,证明EPC1缺失导致H2A泛素化水平下降,影响干细胞分化,提示其在表观遗传调控中的关键作用。

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3. **文献名称**:*EPC1 as a Novel Biomarker for Early-Stage Lung Adenocarcinoma*

**作者**:Chen R, et al.

**摘要**:利用EPC1抗体进行组织微阵列分析,发现EPC1在早期肺腺癌中高表达,与患者预后显著相关,提示其可作为潜在诊断标志物及治疗靶点。

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4. **文献名称**:*Interaction Mapping of EPC1 with Chromatin Remodeling Factors*

**作者**:Gomez F, et al.

**摘要**:通过免疫共沉淀(Co-IP)结合质谱分析,鉴定EPC1与多个染色质重塑因子(如BMI1、SUZ12)的直接互作,阐明了其在维持异染色质稳定性中的分子机制。

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注:以上文献为示例性质,实际研究需参考真实数据库(如PubMed)。如需真实文献,建议通过学术引擎检索关键词“EPC1 antibody”或“EPC1 Polycomb”。

背景信息

The EPC1 (Enhancer of Polycomb 1) antibody is a tool used to study the EPC1 protein, a component of the Polycomb Repressive Complex 2 (PRC2) in eukaryotic cells. EPC1. also known as PHF1 (PHD Finger Protein 1), plays a role in gene silencing by facilitating PRC2-mediated trimethylation of histone H3 on lysine 27 (H3K27me3), a hallmark of transcriptionally repressive chromatin. This protein contains a Tudor domain and a C-terminal PHD finger, enabling interactions with chromatin and other PRC2 subunits like SUZ12 and EZH2. EPC1 is implicated in maintaining cellular identity, regulating developmental genes, and suppressing differentiation in stem cells. Dysregulation of EPC1 has been linked to cancers, including sarcomas and endometrial tumors, where overexpression may drive oncogenic proliferation or epigenetic instability. Researchers use EPC1 antibodies in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and role in chromatin remodeling. These studies contribute to understanding epigenetic mechanisms in development, disease, and potential therapeutic targeting of PRC2-associated pathways.

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