| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACRV1 |
| Uniprot No | P26436 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-265aa |
| 氨基酸序列 | QPNELSGSIDHQTSVQQLPGEFFSLENPSDAEALYETSSGLNTLSEHGSS EHGSSKHTVAEHTSGEHAESEHASGEPAATEHAEGEHTVGEQPSGEQPSG EHLSGEQPLSELESGEQPSDEQPSGEHGSGEQPSGEQASGEQPSGEHASG EQASGAPISSTSTGTILNCYTCAYMNDQGKCLRGEGTCITQNSQQCMLKK IFEGGKLQFMVQGCENMCPSMNLFSHGTRMQIICCRNQSFCNKI |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACRV1重组蛋白的模拟参考文献示例(实际文献需通过学术数据库查询)：
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1. **文献名称**: "Expression and Purification of Recombinant ACRV1 Protein for Immunological Studies"
**作者**: Smith J, et al.
**摘要**: 本研究通过原核表达系统成功克隆并纯化了重组ACRV1蛋白，验证其抗原性可用于抗精子抗体检测。Western blot显示重组蛋白与患者血清特异性结合，为免疫性不育诊断提供工具。
2. **文献名称**: "Structural Characterization of ACRV1 and Its Role in Sperm-Zona Pellucida Interaction"
**作者**: Chen L, et al.
**摘要**: 解析了重组ACRV1蛋白的晶体结构，发现其结构域在精子-透明带结合中起关键作用。体外实验表明ACRV1缺失显著降低精子穿透能力，提示其生殖生物学功能。
3. **文献名称**: "Development of ACRV1-Based Contraceptive Vaccine: Preclinical Evaluation in Animal Models"
**作者**: Gupta R, et al.
**摘要**: 利用重组ACRV1蛋白开发避孕疫苗，动物实验显示可诱导高滴度抗体并显著降低生育率，验证了其作为免疫避孕靶标的潜力，未观察到显著副作用。
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**注意**：以上为模拟文献，实际引用请通过PubMed/Google Scholar检索关键词(如"ACRV1 recombinant protein")。ACRV1相关研究多集中于生殖医学领域，重点关注其在不育症诊断或避孕疫苗中的应用。
**Background of ACRV1 Recombinant Protein**
ACRV1 (Acrosomal Vesicle Protein 1), also known as SP-10. is a sperm-specific protein predominantly localized in the acrosomal matrix and membrane of mature spermatozoa. It plays a critical role in sperm-egg interactions during fertilization, particularly in the acrosome reaction—a process essential for sperm penetration through the zona pellucida of the oocyte. Structurally, ACRV1 contains conserved functional domains, including a zona pellucida-binding region, which facilitates its involvement in gamete recognition and fusion.
The recombinant ACRV1 protein is produced using genetic engineering techniques, typically through expression in heterologous systems like *E. coli* or mammalian cell lines. This allows large-scale production of the protein with high purity and consistency, bypassing ethical and technical challenges associated with isolating native ACRV1 from human sperm. Recombinant ACRV1 retains key biological activities, such as immunogenicity and binding capabilities, making it a valuable tool for studying sperm physiology, infertility mechanisms, and contraceptive development.
Research applications include its use in immunological assays to detect anti-sperm antibodies linked to infertility, as well as in structural studies to map epitopes for contraceptive vaccine design. Additionally, recombinant ACRV1 serves as a standardized antigen in diagnostic kits, improving reproducibility in clinical settings. Despite its utility, challenges remain, such as mimicking post-translational modifications (e.g., glycosylation) critical for native function, necessitating optimization of expression systems. Current studies also explore its potential in elucidating molecular pathways of fertilization and developing targeted therapies for male reproductive disorders.
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