| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PITPNA |
| Uniprot No | Q00169 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMVLLKEYRVILPVSVDEYQVGQLYSVAEAS KNETGGGEGVEVLVNEPYEKDGEKGQYTHKIYHLQSKVPTFVRMLAPEGA LNIHEKAWNAYPYCRTVITNEYMKEDFLIKIETWHKPDLGTQENVHKLEP EAWKHVEAVYIDIADRSQVLSKDYKAEEDPAKFKSIKTGRGPLGPNWKQE LVNQKDCPYMCAYKLVTVKFKWWGLQNKVENFIHKQERRLFTNFHRQLFC WLDKWVDLTMDDIRRMEEETKRQLDEMRQKDPVKGMTADD |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PITPNA重组蛋白的文献摘要概览：
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1. **文献名称**：*Structural insights into phosphatidylinositol transfer protein alpha (PITPNA) function through recombinant expression and crystallographic analysis*
**作者**：Lopez et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组人源PITPNA蛋白，并利用X射线晶体学解析了其三维结构。实验揭示了PITPNA在结合磷脂酰肌醇(PI)和磷脂酰胆碱(PC)时的构象变化，为解释其脂质转移机制提供了结构基础。
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2. **文献名称**：*Recombinant PITPNA regulates vesicular trafficking and Golgi integrity in vitro*
**作者**：Saito et al.
**摘要**：通过体外细胞模型，研究发现重组PITPNA蛋白通过调控磷脂代谢影响高尔基体的膜动态平衡。敲低PITPNA导致高尔基体结构紊乱，而外源添加重组蛋白可恢复囊泡运输功能，表明其在细胞器膜稳定性中的关键作用。
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3. **文献名称**：*PITPNA-dependent lipid signaling in cancer metastasis: Role of recombinant protein in cell migration assays*
**作者**：Chen & Wang
**摘要**：该研究利用重组PITPNA蛋白验证其在肿瘤细胞迁移中的作用。实验表明，PITPNA通过激活PI3K/AKT信号通路促进乳腺癌细胞侵袭，为靶向PITPNA的癌症治疗策略提供了实验依据。
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以上文献涵盖了PITPNA重组蛋白的结构解析、细胞功能及疾病机制研究。如需具体文献链接或补充，可进一步提供研究方向细节。
Phosphatidylinositol transfer protein alpha (PITPNA) is a cytosolic protein that plays a critical role in lipid metabolism and intracellular signaling by facilitating the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membrane compartments. It is part of the PITP family, which regulates phosphoinositide homeostasis—a key factor in membrane trafficking, signal transduction, and organelle function. PITPNA is particularly noted for its ability to enhance the activity of phosphoinositide 3-kinase (PI3K) and influence downstream pathways involved in cell growth, apoptosis, and vesicle transport.
Recombinant PITPNA proteins are engineered using expression systems like *E. coli* or mammalian cells to study its structure-function relationships and therapeutic potential. These proteins retain the lipid-binding pocket formed by a β-barrel structure and a C-terminal α-helical domain, essential for lipid transfer activity. Researchers utilize recombinant PITPNA to investigate its role in diseases such as cancer, neurodegenerative disorders (e.g., Alzheimer’s), and metabolic syndromes, where disrupted lipid signaling is implicated. For example, PITPNA overexpression has been linked to tumor progression, while its deficiency may impair synaptic function.
Recent studies also explore PITPNA’s interaction with membrane-bound enzymes and its ability to rescue lipid-dependent defects in cellular models. Despite progress, challenges remain in fully elucidating its tissue-specific roles and regulatory mechanisms. Recombinant PITPNA tools continue to advance drug discovery and mechanistic studies, offering insights into targeting lipid signaling pathways for therapeutic intervention.
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