| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INPP5A |
| Uniprot No | Q14642 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-410aa |
| 氨基酸序列 | MAGKAAAPGTAVLLVTANVGSLFDDPENLQKNWLREFYQVVHTHKPHFMA LHCQEFGGKNYEASMSHVDKFVKELLSSDAMKEYNRARVYLDENYKSQEH FTALGSFYFLHESLKNIYQFDFKAKKYRKVAGKEIYSDTLESTPMLEKEK FPQDYFPECKWSRKGFIRTRWCIADCAFDLVNIHLFHDASNLVAWETSPS VYSGIRHKALGYVLDRIIDQRFEKVSYFVFGDFNFRLDSKSVVETLCTKA TMQTVRAADTNEVVKLIFRESDNDRKVMLQLEKKLFDYFNQEVFRDNNGT ALLEFDKELSVFKDRLYELDISFPPSYPYSEDARQGEQYMNTRCPAWCDR ILMSPSAKELVLRSESEEKVVTYDHIGPNVCMGDHKPVFLAFRIMPGAGK PHAHVHKCCVDHHHHHH |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant expression and characterization of human INPP5A phosphatase: substrate specificity and kinetic analysis"**
*Authors: Smith J, et al.*
摘要：研究通过大肠杆菌系统重组表达人源INPP5A蛋白，分析其酶活性和底物特异性，发现其对磷脂酰肌醇信号分子具有选择性催化作用，并测定动力学参数。
2. **"Structural insights into INPP5A-mediated phosphatidylinositol phosphate metabolism"**
*Authors: Lee H, et al.*
摘要：通过昆虫细胞表达系统获得重组INPP5A蛋白，结合X射线晶体学解析其三维结构，揭示催化域关键氨基酸残基在底物结合和水解中的分子机制。
3. **"Functional rescue of INPP5A deficiency using recombinant protein therapy in cellular models"**
*Authors: Chen R, et al.*
摘要：利用哺乳动物细胞表达纯化的重组INPP5A蛋白，验证其在INPP5A缺陷细胞中恢复肌醇磷酸代谢稳态的能力，为潜在治疗应用提供依据。
4. **"Development of a high-yield purification protocol for recombinant INPP5A and its application in neurodegenerative disease research"**
*Authors: Gonzalez M, et al.*
摘要：优化HEK293细胞中INPP5A重组蛋白的表达与纯化工艺，获得高纯度蛋白，并证明其在小鼠神经元模型中缓解异常磷脂积累的作用。
(注：以上为模拟文献，实际研究中建议通过PubMed或Google Scholar以“INPP5A recombinant protein”“INPP5A expression”等关键词检索具体文献。)
**Background of INPP5A Recombinant Protein**
INPP5A (Inositol Polyphosphate-5-Phosphatase A), also known as SHIP2 (SH2-containing inositol phosphatase 2), is a phosphatase enzyme encoded by the *INPP5A* gene. It belongs to the inositol polyphosphate 5-phosphatase family, which catalyzes the hydrolysis of phosphate groups at the 5-position of phosphatidylinositol (PI) lipids, particularly phosphatidylinositol 3.4.5-trisphosphate [PtdIns(3.4.5)P3] and phosphatidylinositol 4.5-bisphosphate [PtdIns(4.5)P2]. These lipids are critical second messengers in cellular signaling pathways regulating processes such as cell growth, apoptosis, vesicle trafficking, and insulin signaling.
Recombinant INPP5A protein is engineered through molecular cloning and expressed in heterologous systems (e.g., *E. coli* or mammalian cells) to study its structure, enzymatic activity, and interactions. The protein typically retains functional domains, including a catalytic 5-phosphatase domain and regulatory regions that mediate membrane association or protein-protein interactions. Its activity modulates PI3K/Akt and other signaling cascades, linking it to metabolic disorders (e.g., diabetes), cancer, and neurodegenerative diseases. Dysregulation of INPP5A is associated with insulin resistance and altered endocytosis, highlighting its therapeutic potential.
Researchers use recombinant INPP5A to investigate substrate specificity, develop inhibitors/activators, and explore its role in disease models. Its production ensures a pure, scalable source for biochemical assays, structural studies (e.g., X-ray crystallography), and drug discovery, advancing insights into phosphoinositide metabolism and related pathologies.
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