| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA polymerase kappa, DINB protein, DINP, POLK, DINB1 |
| Entrez GeneID | 51426 |
| WB Predicted band size | 98.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human POLK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于POLK(DNA聚合酶κ)抗体的参考文献示例(内容为假设性概括，建议通过学术数据库核实)：
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1. **文献名称**：*DNA Polymerase κ: A Key Enzyme in Translesion DNA Synthesis*
**作者**：Johnson RE, et al.
**摘要**：该研究鉴定了POLK作为跨损伤合成(TLS)的核心聚合酶，能够绕过紫外线诱导的DNA损伤。文中开发了特异性POLK抗体，用于验证其在细胞核内的定位及与DNA损伤修复复合物的相互作用。
2. **文献名称**：*Mechanistic Insights into POLK-Mediated Error-Prone Bypass of DNA Lesions*
**作者**：Ohashi E, et al.
**摘要**：通过体外实验和细胞模型，揭示了POLK在复制叉停滞时优先插入核苷酸的分子机制。研究使用POLK抗体进行免疫沉淀和Western blot分析，证实其与PCNA(增殖细胞核抗原)的功能关联。
3. **文献名称**：*Regulation of POLK Expression in Human Cancers*
**作者**：Bienko M, et al.
**摘要**：探讨了POLK在肿瘤组织中的异常表达及其与化疗耐药性的关系。研究通过免疫组化(使用POLK抗体)显示，POLK高表达与卵巢癌患者预后不良显著相关。
4. **文献名称**：*A Novel Antibody-Based Assay for Quantifying POLK Activity in Vivo*
**作者**：Sale JE, et al.
**摘要**：开发了一种基于POLK抗体的ELISA检测方法，用于定量分析细胞或组织中POLK的活性水平，为评估DNA修复能力提供了新工具。
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**备注**：以上文献信息为示例，实际研究需通过PubMed、Web of Science等平台检索关键词“POLK antibody”“DNA polymerase kappa”等确认。
POLK antibody targets DNA polymerase kappa (Polκ), a Y-family DNA polymerase involved in translesion synthesis (TLS), a critical DNA damage tolerance mechanism. Discovered in the early 2000s, Polκ enables replication past DNA lesions that stall high-fidelity polymerases, preventing replication fork collapse. It specializes in bypassing bulky adducts, such as those induced by environmental carcinogens (e.g., benzo[a]pyrene), but exhibits low processivity and fidelity compared to replicative polymerases. Dysregulation of Polκ is linked to genomic instability, mutagenesis, and carcinogenesis.
Polκ's role in cancer is dual: while its error-prone activity may promote tumorigenesis, it also aids cancer cell survival under genotoxic stress, contributing to chemotherapy resistance. Overexpression of Polκ has been observed in various cancers (e.g., ovarian, lung, and breast), often correlating with poor prognosis. POLK antibodies are essential tools for studying its expression, localization, and interactions in DNA repair pathways. Clinically, they aid in evaluating Polκ as a potential biomarker for cancer risk stratification or therapeutic targeting. Current research explores inhibiting Polκ to sensitize tumors to DNA-damaging chemotherapies, highlighting its dual significance in genome maintenance and cancer therapy.
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