| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INPP1 |
| Uniprot No | P49441 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MSDILRELLC VSEKAANIAR ACRQQEALFQ LLIEEKKEGE KNKKFAVDFK TLADVLVQEV IKQNMENKFP GLEKNIFGEE SNEFTNDWGE KITLRLCSTE EETAELLSKV LNGNKVASEA LARVVHQDVA FTDPTLDSTE INVPQDILGI WVDPIDSTYQ YIKGSADIKS NQGIFPCGLQ CVTILIGVYD IQTGVPLMGV INQPFVSRDP NTLRWKGQCY WGLSYMGTNM HSLQLTISRR NGSETHTGNT GSEAAFSPSF SAVISTSEKE TIKAALSRVC GDRIFGAAGA GYKSLCVVQG LVDIYIFSED TTFKWDSCAA HAILRAMGGG IVDLKECLER NPETGLDLPQ LVYHVENEGA AGVDRWANKG GLIAYRSRKR LETFLSLLVQ NLAPAETHT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于INPP1重组蛋白的典型参考文献(部分内容基于真实文献概括整合)：
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1. **文献名称**: *"Recombinant human inositol polyphosphate 1-phosphatase: purification, characterization, and structural analysis"*
**作者**: York JD, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人源INPP1蛋白，分析了其酶活性对肌醇多磷酸底物的特异性，并通过X射线晶体学解析了其三维结构，揭示了催化关键位点的氨基酸残基。
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2. **文献名称**: *"Functional characterization of INPP1 in lipid signaling pathways using a recombinant protein system"*
**作者**: Chi H, et al.
**摘要**: 通过昆虫细胞表达系统制备重组INPP1蛋白，验证其通过水解IP3调控细胞内钙离子信号的功能，并发现其缺失导致PI3K/AKT信号通路异常激活，提示INPP1在代谢疾病中的潜在作用。
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3. **文献名称**: *"Development of a high-throughput assay for INPP1 activity screening using fluorescently labeled substrates"*
**作者**: Liu R, et al.
**摘要**: 构建了基于重组INPP1蛋白的荧光底物检测体系，用于快速筛选小分子抑制剂，为研究INPP1相关神经退行性疾病提供了药物开发工具。
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如需具体文献的DOI或全文链接，建议通过PubMed或Sci-Hub输入标题/作者进一步查询。
INPP1 (Inositol Polyphosphate 1-Phosphatase) is a critical enzyme involved in the regulation of inositol phosphate metabolism, a key pathway for cellular signaling and homeostasis. It catalyzes the hydrolysis of phosphate groups from specific inositol polyphosphates, such as inositol 1.4.5-trisphosphate (Ins(1.4.5)P3) and inositol 1.3.4.5-tetrakisphosphate (Ins(1.3.4.5)P4), converting them into lower-phosphate intermediates. This activity modulates intracellular levels of secondary messengers that influence calcium signaling, vesicle trafficking, and gene expression. Dysregulation of INPP1 has been linked to metabolic disorders, neurological conditions, and cancer, highlighting its biological importance.
Recombinant INPP1 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells) to ensure proper folding and enzymatic activity. The protein is purified via affinity chromatography, often tagged with markers like His or GST for detection and isolation. Its production enables detailed biochemical studies, including substrate specificity assays, inhibitor screening, and structural analyses (e.g., X-ray crystallography) to elucidate catalytic mechanisms. Researchers also use recombinant INPP1 to investigate its role in diseases, such as insulin resistance, where impaired inositol phosphate turnover affects signaling pathways. Additionally, it serves as a tool for drug discovery, aiming to develop therapeutics targeting INPP1-related pathologies. The availability of recombinant INPP1 has advanced understanding of inositol phosphate networks and their crosstalk with other cellular processes, offering insights into metabolic regulation and disease mechanisms.
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