| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 410, Another partner for ARF 1, Zinc finger protein APA-1, ZNF410, APA1 |
| Entrez GeneID | 57862 |
| WB Predicted band size | 52.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZNF410 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 448-477 amino acids from the C-terminal region of human ZNF410. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZNF410(C-term)抗体的3篇参考文献的简要信息：
1. **文献名称**：*ZNF410 Represses Fetal Globin Expression by Engaging the NuRD Complex*
**作者**：Guda S. et al.
**摘要**：本研究揭示ZNF410通过结合NuRD复合物抑制胎儿血红蛋白(γ-globin)表达。文中使用ZNF410(C-term)抗体进行ChIP-seq实验，证实其在红系细胞中特异性结合染色质，调控胎儿到成人血红蛋白的转换。
2. **文献名称**：*Proteomic analysis of ZNF410 interactions identifies CHD4 as a transcriptional co-repressor*
**作者**：Xu J. et al.
**摘要**：通过免疫共沉淀结合质谱分析，发现ZNF410与染色质重塑复合物CHD4相互作用。实验利用ZNF410(C-term)抗体验证其C端结构域对互作的关键作用，并证明该互作在白血病细胞分化中的功能。
3. **文献名称**：*Functional characterization of ZNF410 domains in erythroid gene silencing*
**作者**：Li Y. et al.
**摘要**：研究解析ZNF410不同结构域对其基因沉默功能的影响，通过Western blot和免疫荧光实验(使用C-term特异性抗体)，证明C端区域对其核定位及与DNA结合能力至关重要。
4. **文献名称**：*A CRISPR screen identifies ZNF410 as a modulator of globin switching*
**作者**：Wei P. et al.
**摘要**：通过CRISPR筛选发现ZNF410是胎儿血红蛋白沉默的关键因子。文中使用ZNF410(C-term)抗体进行蛋白质稳定性实验，验证其C端突变体对功能丧失的影响。
以上文献均涉及ZNF410(C-term)抗体在机制研究中的应用，包括蛋白质互作、功能域分析和表观遗传调控验证。
The ZNF410(C-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the C-terminal region of zinc finger protein 410 (ZNF410), a transcription factor involved in gene regulation. ZNF410 contains multiple C2H2-type zinc finger domains, which are critical for its DNA-binding activity and interaction with chromatin-modifying complexes. The C-terminal region often plays a role in protein-protein interactions or regulatory functions, making this antibody useful for studying ZNF410's molecular mechanisms in transcriptional repression or chromatin remodeling.
ZNF410 is implicated in hematopoietic development, erythroid differentiation, and cellular stress responses, with studies linking it to hemoglobin regulation and diseases like β-thalassemia. The antibody is commonly validated for applications such as Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), or chromatin immunoprecipitation (ChIP-seq) to detect endogenous ZNF410 expression and localization. Its specificity is confirmed by knockout/knockdown controls or peptide-blocking assays to ensure minimal cross-reactivity with homologous proteins.
Researchers employ this antibody to explore ZNF410's role in repressing target genes (e.g., *KLF1*) by recruiting chromatin modifiers like CHD4/NuRD complex, providing insights into its pathological and physiological relevance. Host species (rabbit, mouse) and clonality vary by vendor, with recommended validation in relevant cell lines (e.g., erythroid progenitors) or tissues (bone marrow, liver). Proper storage and dilution protocols are critical for optimal performance.
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