| 纯度 | ＞90% by SDS-PAGE. |
| 种属 | Human |
| 靶点 | YWHAH |
| Uniprot No | Q04917 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4-246aa |
| 氨基酸序列 | REQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN |
| 预测分子量 | 51.9 kDa |
| 蛋白标签 | C-his |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YWHAH重组蛋白的3篇文献摘要示例(注：文献为虚构示例，实际文献需通过学术数据库检索)：
1. **文献名称**: "Expression and purification of recombinant YWHAH/14-3-3η protein for structural studies"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了通过大肠杆菌表达系统高效表达YWHAH重组蛋白的方法，采用亲和层析和凝胶过滤技术纯化获得高纯度蛋白，并通过圆二色光谱验证其二级结构完整性，为后续互作蛋白结合实验提供了材料基础。
2. **文献名称**: "YWHAH regulates apoptosis via interaction with BAD protein: Insights from recombinant protein assays"
**作者**: Kim S, Park JH
**摘要**: 利用重组YWHAH蛋白与BAD蛋白的体外结合实验，结合表面等离子共振技术(SPR)，证明YWHAH通过特异性磷酸化依赖的方式调控BAD的促凋亡功能，揭示了其在细胞死亡通路中的分子机制。
3. **文献名称**: "Crystallographic analysis of human YWHAH in complex with a phosphopeptide ligand"
**作者**: Müller R, et al.
**摘要**: 通过重组表达人源YWHAH蛋白并解析其与含磷酸化基序的肽段复合物的晶体结构，阐明了14-3-3η蛋白的底物结合口袋特征及磷酸化依赖的相互作用模式，为靶向药物设计提供了结构依据。
4. **文献名称**: "Development of a YWHAH-based biosensor for detecting kinase activity in vitro"
**作者**: Chen X, et al.
**摘要**: 基于重组YWHAH蛋白的构象变化特性，构建了荧光共振能量转移(FRET)生物传感器，成功应用于实时监测多种激酶的活性，证明其在体外诊断工具开发中的潜在价值。
**提示**：实际文献可通过PubMed、Web of Science以关键词"YWHAH recombinant"或"14-3-3 eta recombinant"检索，建议优先选择近五年内发表的蛋白质表达、结构功能或疾病机制相关研究。
**Background of YWHAH Recombinant Protein**
YWHAH, also known as 14-3-3 eta (η), is a member of the evolutionarily conserved 14-3-3 protein family, which comprises seven isoforms in humans. These proteins function as adaptors or scaffolds, regulating diverse cellular processes by binding to phosphorylated client proteins. YWHAH is encoded by the *YWHAH* gene and is highly expressed in the brain, skeletal muscle, and other tissues, playing critical roles in signal transduction, cell cycle control, apoptosis, and metabolism.
Recombinant YWHAH protein is produced in vitro using expression systems such as *E. coli* or mammalian cell lines, enabling precise study of its structure and interactions. Its primary structure includes a conserved ligand-binding groove that recognizes phosphorylated serine/threonine motifs in target proteins, such as kinases, transcription factors, and apoptotic regulators. This binding modulates client protein localization, stability, or activity, influencing pathways like PI3K/AKT, MAPK, and mTOR.
YWHAH has been implicated in neurodegenerative diseases (e.g., Alzheimer’s), cancer, and autoimmune disorders. For instance, elevated YWHAH levels correlate with poor prognosis in cancers due to its anti-apoptotic effects, while in rheumatoid arthritis, it serves as a biomarker. Recombinant YWHAH is vital for elucidating these mechanisms, screening therapeutic agents, and developing diagnostic tools.
Current research focuses on resolving its tissue-specific roles and isoform-selective functions. Challenges include deciphering its complex interaction networks and exploring its potential as a drug target. Recombinant YWHAH, often tagged for purification (e.g., His-tag), remains a key tool for advancing molecular and therapeutic studies.
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